Suppressor of Variegation 3-9 Homolog 1 (Drosophila) (SUV39H1) antibody

Antigen: Suppressor of Variegation 3-9 Homolog 1 (Drosophila) (SUV39H1)

Synonyms: MG44, KMT1A, SUV39H

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN387991

Additional Information

Alternative name: SUV39H1

Gene ID: 6839

UniProt: NP_003164

Immunogen: Purified recombinant GST fusion protein encoding N-terminal of human SUV39H1.

Isotype: Ig

Description: Other names: suppressor of variegation 3-9 homolog 1, Histone H3-K9 methyltransferase 1, H3-K9-HMTase 1, Su(var)3-9 homolog 1, KMT1A

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: Purified recombinant GST fusion protein encoding N-terminal of human SUV39H1.

Molecular Weight: 47907 DA

Comments:

Background: Similar to acetylation and phosphorylation, histone methylation at the N-terminal tail has emerged as an important role in regulating chromatin dynamics and gene activity. Histone methylation occurs on arginine and lysine residues and is catalyzed by two families of proteins, the protein arginine methyltransferase family and the SET-domain-containing methyltransferase family. Five members have been identified in the arginine methyltransferase family. About 27 are grouped into the SET-domain family, and another 17 make up the PR domain family that is related to the SET domain family. The retinoblastoma protein-interacting zinc finger geneRIZ1 is a tumor suppressor gene and a FOUNDING member of the PR domain family. RIZ1 inactivation is commonly found in many types of human cancers and occurs through loss of mRNA expression, frame shift mutation, chromosomal deletion, and missense mutation. RIZ1 is also a tumor susceptibility gene in mice. The loss of RIZ1 mRNA in human cancers was shown to associate with DNA methylation of its promoter CpG island. Methylation of the RIZ1 promoter strongly correlated with lost or decreased RIZ1 mRNA expression in breast, liver, colon, and lung cancer cell lines as well as in liver cancer tissues.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications, Cancer, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

SUV39H1 (ABIN387991) western blot analysis in MDA-MB435 cell line lysates (35ug/lane). This demonstrates the SUV39H1 antibody detected the SUV39H1 protein (arrow).

Publications

Product: Rea, Eisenhaber, OCarroll et al.: "Regulation of chromatin structure by site-specific histone H3 methyltransferases." in: Nature, Vol. 406, Issue 6796, pp. 593-9, 2000 (PubMed).

Vaute, Nicolas, Vandel et al.: "Functional and physical interaction between the histone methyl transferase Suv39H1 and histone deacetylases." in: Nucleic acids research, Vol. 30, Issue 2, pp. 475-81, 2002 (PubMed).

Schotta, Ebert, Krauss et al.: "Central role of Drosophila SU(VAR)3-9 in histone H3-K9 methylation and heterochromatic gene silencing." in: The EMBO journal, Vol. 21, Issue 5, pp. 1121-31, 2002 (PubMed).

Fujita, Watanabe, Ichimura et al.: "Methyl-CpG binding domain 1 (MBD1) interacts with the Suv39h1-HP1 heterochromatic complex for DNA methylation-based transcriptional repression." in: The Journal of biological chemistry, Vol. 278, Issue 26, pp. 24132-8, 2003 (PubMed).

Macaluso, Cinti, Russo et al.: "pRb2/p130-E2F4/5-HDAC1-SUV39H1-p300 and pRb2/p130-E2F4/5-HDAC1-SUV39H1-DNMT1 multimolecular complexes mediate the transcription of estrogen receptor-alpha in breast cancer." in: Oncogene, Vol. 22, Issue 23, pp. 3511-7, 2003 (PubMed).