SET Domain Containing (Lysine Methyltransferase) 8 (SETD8) (N-Term) antibody

Antigen: SET Domain Containing (Lysine Methyltransferase) 8 (SETD8)

Synonyms: PR-SET7, AA617402, AW536475, MGC117832, 2410195B05Rik, SET8, KMT5A, SET07, PR-Set7

Epitope: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN387992

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: SET07 (N-term)

Gene ID: 387893

Swiss-Prot: Q9NQR1

Immunogen: This SET07 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32~62 amino acids from the N-terminal region of human SET07.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: Histone-lysine N-methyltransferase, H4 lysine-20 specific, Histone H4-K20 methyltransferase, H4-K20-HMTase, SET domain-containing protein 8, PR/SET domain-containing protein 07, PR-Set7 , PR/SET07, KMT5A

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human SET07.

Molecular Weight: 42890 DA

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications, Cancer, Transcription Factors, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of SET07 polyclonal antibody (ABIN387992) in HeLa cell lysate (Lane 1) and NIH/3T3 cell lysate (Lane 2). SET07 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.