SET Domain Containing (Lysine Methyltransferase) 8 (SETD8) (N-Term) antibody

Antigen: SET Domain Containing (Lysine Methyltransferase) 8 (SETD8)

Synonyms: PR-SET7, AA617402, AW536475, MGC117832, 2410195B05Rik, SET8, KMT5A, SET07, PR-Set7

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN387992

Additional Information

Alternative name: SET07

Gene ID: 387893

UniProt: Q9NQR1

Immunogen: This SET07 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32~62 amino acids from the N-terminal region of human SET07.

Isotype: Ig

Description: Other names: Histone-lysine N-methyltransferase, H4 lysine-20 specific, Histone H4-K20 methyltransferase, H4-K20-HMTase, SET domain-containing protein 8, PR/SET domain-containing protein 07, PR-Set7 , PR/SET07, KMT5A

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This SET07 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32~62 amino acids from the N-terminal region of human SET07.

Molecular Weight: 42890 DA

Comments:

Background: SET07 is a histone methyltransferase that methylates Lys-20 of histone H4. H4 Lys-20 methylation represents a specific tag for epigenetic transcriptional repression. The nuclear SET07 protein, which associates with silent chromatin on euchromatic arms but shows no association with constitutive heterochromatin, prefers nucleosomes as substrate compared to free histones. It appears that SET07 may play a role in maintaining silent chromatin by preventing neighboring acetylation of H4 tail. Although the SET domain contains the active site of enzymatic activity, both sequences upstream and downstream of the SET domain are required for methyltransferase activity.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications, Cancer, Transcription Factors, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of SET07 polyclonal antibody (ABIN387992) in HeLa cell lysate (Lane 1) and NIH/3T3 cell lysate (Lane 2). SET07 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Nishioka, Rice, Sarma et al.: "PR-Set7 is a nucleosome-specific methyltransferase that modifies lysine 20 of histone H4 and is associated with silent chromatin." in: Molecular cell, Vol. 9, Issue 6, pp. 1201-13, 2002 (PubMed).