Lysine (K)-Specific Demethylase 1A (KDM1A) (C-Term) antibody

Antigen: Lysine (K)-Specific Demethylase 1A (KDM1A)

Synonyms: AOF2, KDM1, LSD1, BHC110, KIAA0601, RP1-184J9.1, Aof2, Lsd1, AA408884, mKIAA0601, D4Ertd478e, 1810043O07Rik, RGD1562975, aof2, wu:fj36a05, KDM1A, DKFZp459N2450, kdm1, lsd1, xLSD1

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388023

Additional Information

Alternative name: LSD1

Gene ID: 23028

UniProt: O60341

Immunogen: This LSD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human LSD1.

Isotype: Ig

Description: Other names: Lysine-specific histone demethylase 1, Amine oxidase flavin containing domain protein 2, BRAF35-HDAC complex protein BHC110, AOF2, KIAA0601, KDM1

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This LSD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human LSD1.

Molecular Weight: 92903 DA

Comments:

Background: LSD1 is a histone demethylase that specifically demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. LSD1 contains a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. It acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. LSD1 demethylates both mono- and tri-methylted 'Lys-4' of histone H3. This protein may play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 'Lys-4' on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. It may also demethylate 'Lys-9' of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes.

Application Details

Application Notes: ELISA~~1:1,000 Western BLot ~~ 1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications, Cancer, Transcription Factors, Chromatin, Neurology, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of AOF2 (arrow) using LSD1 Antibody (C-term) (ABIN388023). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AOF2 gene (Lane 2) (Origene Technologies).

Western immunoblot. Nuclear extracts of control and PhIP-treated HMEC. Proteins were transferred onto polyvinylidene difluoride and blotted with anti-LSD1 antibody. Nuclear LSD1 protein levels increased in carcinogen-treated HMEC compared with control HMEC.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Humphrey, Wang, Russanova et al.: "Stable histone deacetylase complexes distinguished by the presence of SANT domain proteins CoREST/kiaa0071 and Mta-L1." in: The Journal of biological chemistry, Vol. 276, Issue 9, pp. 6817-24, 2001 (PubMed).

Hakimi, Bochar, Chenoweth et al.: "A core-BRAF35 complex containing histone deacetylase mediates repression of neuronal-specific genes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 11, pp. 7420-5, 2002 (PubMed).

Hakimi, Dong, Lane et al.: "A candidate X-linked mental retardation gene is a component of a new family of histone deacetylase-containing complexes." in: The Journal of biological chemistry, Vol. 278, Issue 9, pp. 7234-9, 2003 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Shi, Lan, Matson et al.: "Histone demethylation mediated by the nuclear amine oxidase homolog LSD1." in: Cell, Vol. 119, Issue 7, pp. 941-53, 2004 (PubMed).

Forneris, Binda, Vanoni et al.: "Histone demethylation catalysed by LSD1 is a flavin-dependent oxidative process." in: FEBS letters, Vol. 579, Issue 10, pp. 2203-7, 2005 (PubMed).