|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Lysine (K)-Specific Demethylase 1A (KDM1A) (C-Term) antibody
|Synonyms||AOF2, KDM1, LSD1, BHC110, KIAA0601, RP1-184J9.1, Aof2, Lsd1, AA408884, mKIAA0601, D4Ertd478e, 1810043O07Rik, RGD1562975, aof2, wu:fj36a05, KDM1A, DKFZp459N2450, kdm1, lsd1, xLSD1|
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), ELISA
|6 references available|
|Quantity||400 µL (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This LSD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human LSD1.|
|Description||Other names: Lysine-specific histone demethylase 1, Amine oxidase flavin containing domain protein 2, BRAF35-HDAC complex protein BHC110, AOF2, KIAA0601, KDM1|
|Characteristics||Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This LSD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human LSD1.|
|Molecular Weight||92903 DA|
Background: LSD1 is a histone demethylase that specifically demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. LSD1 contains a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. It acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. LSD1 demethylates both mono- and tri-methylted 'Lys-4' of histone H3. This protein may play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 'Lys-4' on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. It may also demethylate 'Lys-9' of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes.
|Application Notes||ELISA~~1:1,000 Western BLot ~~ 1:100~500 Immunohistochemistry~~1:50~100|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Signaling, Protein Modifications, Cancer, Transcription Factors, Chromatin, Neurology, Cell Structure|
|Restrictions||For Research Use only|
Humphrey, Wang, Russanova et al.: "Stable histone deacetylase complexes distinguished by the presence of SANT domain proteins CoREST/kiaa0071 and Mta-L1." in: The Journal of biological chemistry, Vol. 276, Issue 9, pp. 6817-24, 2001 (PubMed).
Hakimi, Bochar, Chenoweth et al.: "A core-BRAF35 complex containing histone deacetylase mediates repression of neuronal-specific genes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 11, pp. 7420-5, 2002 (PubMed).
Hakimi, Dong, Lane et al.: "A candidate X-linked mental retardation gene is a component of a new family of histone deacetylase-containing complexes." in: The Journal of biological chemistry, Vol. 278, Issue 9, pp. 7234-9, 2003 (PubMed).
Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).
Shi, Lan, Matson et al.: "Histone demethylation mediated by the nuclear amine oxidase homolog LSD1." in: Cell, Vol. 119, Issue 7, pp. 941-53, 2004 (PubMed).
Forneris, Binda, Vanoni et al.: "Histone demethylation catalysed by LSD1 is a flavin-dependent oxidative process." in: FEBS letters, Vol. 579, Issue 10, pp. 2203-7, 2005 (PubMed).
|Hosts||Rabbit (43), Mouse (19)|
|Reactivities||Human (55), Mouse (Murine) (16), Rat (Rattus) (13), Dog (Canine) (5), Cow (Bovine) (4), Monkey (2), Cat (Feline) (1), Chicken (1), Mammalian (1), Pig (Porcine) (1), Primate (1), Rabbit (1)|
|Applications||Western Blotting (WB) (63), ELISA (35), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (23), Immunohistochemistry (IHC) (11), Immunoprecipitation (IP) (6), Immunofluorescence (IF) (5), Immunocytochemistry (ICC) (4), Immunohistochemistry (Fixed) (IHC (fx)) (3), Dot Blot (Dot) (1), Flow Cytometry (FACS) (1), Immunoassay (IA) (1)|
|Epitopes||N-Term (9), Internal Region (4), AA 100-150 (3), AA 800-850 (3), C-Term (3), AA 400-450 (2), AA 450-500 (2), Center (2), AA 1-50 (1), AA 50-100 (1), Middle Region (1)|