PIASy1 (PIASy1) (C-Term) antibody

Antigen: PIASy1 (PIASy1)

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388062

Additional Information

Alternative name: PIASy1

Gene ID: 51588

UniProt: Q8N2W9

Immunogen: This PIASy1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 478~507 amino acids from the C-terminal region of human PIASy1.

Isotype: Ig

Description: Other names: E3 SUMO-protein ligase PIAS4, Protein inhibitor of activated STAT protein 4, Protein inhibitor of activated STAT protein gamma, PIAS-gamma, PIASy, PIAS4, PIASG

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This PIASy1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 478~507 amino acids from the C-terminal region of human PIASy1.

Molecular Weight: 56504 DA

Comments:

Background: PIASy1 functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. This protein plays a crucial role in transcriptional coregulation of various cellular pathways, including the STAT pathway, the p53 pathway, the wnt pathway and the steroid hormone signaling pathway. PIASy1 is involved in gene silencing, and promotes PARK7 sumoylation.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Apoptosis/Necrosis, Cell Signaling, Protein Modifications, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

The PIASy1 polyclonal antibody (ABIN388062) is used in Western blot to detect recombinant GST-PIASy1 in bacterial cell lysate.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Liu, Liao, Rao et al.: "Inhibition of Stat1-mediated gene activation by PIAS1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 18, pp. 10626-31, 1998 (PubMed).

Liu, Gross, ten Hoeve et al.: "A transcriptional corepressor of Stat1 with an essential LXXLL signature motif." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 6, pp. 3203-7, 2001 (PubMed).

Imoto, Sugiyama, Muromoto et al.: "Regulation of transforming growth factor-beta signaling by protein inhibitor of activated STAT, PIASy through Smad3." in: The Journal of biological chemistry, Vol. 278, Issue 36, pp. 34253-8, 2003 (PubMed).