|+1 404 474 4654|
|+1 888 205 9894 (TF)|
SUMO/sentrin Specific Peptidase Family Member 8 (SENP8) (N-Term) antibody
|Synonyms||DEN1, NEDP1, PRSC2, HsT17512, den1, nedp1, prsc2, MGC84956|
Alternatives Western Blotting (WB), ELISA
|3 references available|
|Quantity||400 µL (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This SENP8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12~42 amino acids from the N-terminal region of human SENP8.|
|Description||Other names: Sentrin-specific protease 8, Sentrin/SUMO-specific protease SENP8, Protease, cysteine 2, NEDD8-specific protease 1, deneddylase-1, DEN1, NEDP1, PRSC2|
|Characteristics||Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This SENP8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12~42 amino acids from the N-terminal region of human SENP8.|
|Molecular Weight||24107 DA|
Background: NEDD8 is a ubiquitin-like protein that becomes conjugated to the cullin subunit of several ubiquitin ligases. This conjugation, called neddylation, is required for optimal ubiquitin ligase activity. SENP8 is a protease that catalyzes two essential functions in the NEDD8 pathway: processing of full-length NEDD8 to its mature form and deconjugation of NEDD8 from targeted proteins such as cullins or p53.
|Application Notes||The suggested dilution will be: ELISA~~1:1,000 Western blotting~~1:100~500|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca|
|Research Area||Apoptosis/Necrosis, Cell Signaling, Protein Modifications, Cell Structure|
|Restrictions||For Research Use only|
Mendoza, Shen, Botting et al.: "NEDP1, a highly conserved cysteine protease that deNEDDylates Cullins." in: The Journal of biological chemistry, Vol. 278, Issue 28, pp. 25637-43, 2003 (PubMed).
Gan-Erdene, Nagamalleswari, Yin et al.: "Identification and characterization of DEN1, a deneddylase of the ULP family." in: The Journal of biological chemistry, Vol. 278, Issue 31, pp. 28892-900, 2003 (PubMed).
Wu, Yamoah, Dolios et al.: "DEN1 is a dual function protease capable of processing the C terminus of Nedd8 and deconjugating hyper-neddylated CUL1." in: The Journal of biological chemistry, Vol. 278, Issue 31, pp. 28882-91, 2003 (PubMed).
|Reactivities||Human (11), Rat (Rattus) (3), Mouse (Murine) (2)|
|Applications||ELISA (10), Western Blotting (WB) (8), Immunohistochemistry (IHC) (2), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (2)|
|Epitopes||C-Term (3), N-Term (2), AA 196-211 (1)|