SUMO/sentrin Specific Peptidase Family Member 8 (SENP8) (N-Term) antibody

Antigen: SUMO/sentrin Specific Peptidase Family Member 8 (SENP8)

Synonyms: DEN1, NEDP1, PRSC2, HsT17512, den1, nedp1, prsc2, MGC84956

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388073

Additional Information

Alternative name: SENP8

Gene ID: 123228

UniProt: Q96LD8

Immunogen: This SENP8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12~42 amino acids from the N-terminal region of human SENP8.

Isotype: Ig

Description: Other names: Sentrin-specific protease 8, Sentrin/SUMO-specific protease SENP8, Protease, cysteine 2, NEDD8-specific protease 1, deneddylase-1, DEN1, NEDP1, PRSC2

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This SENP8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12~42 amino acids from the N-terminal region of human SENP8.

Molecular Weight: 24107 DA

Comments:

Background: NEDD8 is a ubiquitin-like protein that becomes conjugated to the cullin subunit of several ubiquitin ligases. This conjugation, called neddylation, is required for optimal ubiquitin ligase activity. SENP8 is a protease that catalyzes two essential functions in the NEDD8 pathway: processing of full-length NEDD8 to its mature form and deconjugation of NEDD8 from targeted proteins such as cullins or p53.

Application Details

Application Notes: The suggested dilution will be: ELISA~~1:1,000 Western blotting~~1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Apoptosis/Necrosis, Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of SENP8 polyclonal antibody (ABIN388073) in mouse spleen lysate. SENP8 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Western blot analysis of SENP8 (arrow) using rabbit polyclonal SENP8 Antibody (S19) (ABIN388073). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SENP8 gene.

Publications

Product: Mendoza, Shen, Botting et al.: "NEDP1, a highly conserved cysteine protease that deNEDDylates Cullins." in: The Journal of biological chemistry, Vol. 278, Issue 28, pp. 25637-43, 2003 (PubMed).

Gan-Erdene, Nagamalleswari, Yin et al.: "Identification and characterization of DEN1, a deneddylase of the ULP family." in: The Journal of biological chemistry, Vol. 278, Issue 31, pp. 28892-900, 2003 (PubMed).

Wu, Yamoah, Dolios et al.: "DEN1 is a dual function protease capable of processing the C terminus of Nedd8 and deconjugating hyper-neddylated CUL1." in: The Journal of biological chemistry, Vol. 278, Issue 31, pp. 28882-91, 2003 (PubMed).