Guanine Nucleotide Binding Protein-Like 3 (Nucleolar) (E2IG3) (C-Term) antibody

Antigen: Guanine Nucleotide Binding Protein-Like 3 (Nucleolar) (E2IG3)

Synonyms: NS, E2IG3, C77032, MGC800, Ns, BC037996, MGC46970, nstm, MGC123093, id:ibd2914, wu:fb38a04, wu:fc55d07, zgc:123093, gnl3, GNL3

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388154

Additional Information

Alternative name: Nucleostemin (GNL3)

Gene ID: 26354

UniProt: Q9BVP2

Immunogen: This GNL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 465~495 amino acids from the C-term region of human GNL3.

Isotype: Ig

Description: Other names: Guanine nucleotide-binding protein-like 3, Nucleolar GTP-binding protein 3, Nucleostemin, E2-induced gene 3 protein, Novel nucleolar protein 47, NNP47, E2IG3, NS

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This GNL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 465~495 amino acids from the C-term region of human GNL3.

Molecular Weight: 61997 DA

Comments:

Background: GNL3 is found in the nucleoli of embryonic stem cells, adult CNS stem cells, primitive cells in the bone marrow and cancer cells. It is not in the differentiated cells of most adult tissues. It has been suggested to play a role in controlling the cell-cycle progression in stem cells and cancer cells, and may be required to maintain the proliferative capacity of stem cells.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western Blot~~1:50~100 Immunohistochemistry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells, Cell Cycle, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of GNL3 Antibody (C-term) (ABIN388154) in HL60 cell line lysates (35ug/lane). GNL3 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with GNL3 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Ma, Pederson: "Depletion of the nucleolar protein nucleostemin causes G1 cell cycle arrest via the p53 pathway." in: Molecular biology of the cell, Vol. 18, Issue 7, pp. 2630-5, 2007 (PubMed).