LC3 (APG8A) (N-term) antibody

Antigen: LC3 (APG8A) (N-term)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388464

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Gene ID: 84557

Swiss-Prot: Q9H492

Immunogen: This LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-term of human LC3 (APG8a).

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: MAP1LC3A, microtubule-associated protein 1 light chain 3 alpha, RP11-346K17.1, LC3, LC3A, MAP1ALC3, MAP1BLC3, MAP1 light chain 3-like protein 1, MAP1A/1B light chain 3 A, MAP1A/MAP1B LC3 A, MAP1A/MAP1B light chain 3 A, OTTHUMP00000030698, autophagy-relate

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide at the N-term of human LC3 (APG8a).

Molecular Weight: 14272 DA

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100 Immunofluorescent~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Cell Structure, Autophagy

Restrictions: For Research Use only

Images

Western blot analysis of anti-LC3 (APG8a) Pab (ABIN388464) in rat brain lysate. Both lipidated (arrow, II) and non-lipidated APG8a (arrow, I) were detected in membrane fraction (P) but only non-lipidated LC3 was detected in soluble fraction (S).

Immunofluorescent (IF) staining of mouse brain cells using anti-LC3 (APG8a) Pab (ABIN388464).

LC3 and the CLN3-encoded protein, battenin, co-immunostaining. Wild-type and mutant cerebellar cells were costained with #ABIN388464 anti-LC3 (APG8a) Abgent antibody (Red) and a monoclonal anti-battenin antibody(Green) and images were captured by confocal microscopy. Note the numerous larger vesicular structures typical of mature Avs stained with both the LC3 and battenin antibodies in wild-type cells (arrows) which are rare in mutant cells. Blue=DAPI.

Publications

Publications: English, Chemali, Duron et al.: "Autophagy enhances the presentation of endogenous viral antigens on MHC class I molecules during HSV-1 infection." in: Nature immunology, Vol. 10, Issue 5, pp. 480-7, 2009 (PubMed).

Cirstea, Hideshima, Rodig et al.: "Dual inhibition of akt/mammalian target of rapamycin pathway by nanoparticle albumin-bound-rapamycin and perifosine induces antitumor activity in multiple myeloma." in: Molecular cancer therapeutics, Vol. 9, Issue 4, pp. 963-75, 2010 (PubMed).