APG8b (MAP1LC3B) (Center), (Thr93), (Tyr99) antibody

Details for Product No. ABIN388476
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Antigen
Epitope
Center, Thr93, Tyr99
(1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(4), (1)
Host
Rabbit
(4)
Clonality
Polyclonal
Application
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
(3), (1), (1), (1)
Pubmed 6 references available
Catalog no. ABIN388476
Quantity 400 µL
Price
291.50 $   Plus shipping costs $45.00
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Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen This APG8b (MAP1LC3B)-T93/Y99 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 81-111 amino acids from the central region of human PMAT-Y198.
Specificity This APG8b (MAP1LC3B)-T93/Y99 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 81-111 amino acids from the central region of human PMAT-Y198.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Background The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.
Synonyms: MAP1LC3B,MAP1ALC3
Molecular Weight 14688 DA
Gene ID 81631
UniProt Q9GZQ8, NP_073729.1
Research Area Cell Structure, Autophagy
Application Notes WB = 1:1000, IHC = 1:50-100, FACS = 1:10-50
Comment

Background: The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.

Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/ml
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-APG8b (MAP1LC3B) (Center), (Thr93), (Tyr99) antibody Western blot analysis of APG8b (MAP1LC3B) Antibody (T93/Y99) (ABIN388476) in MCF-7 cell line lysates (35 µg/lane). MAP1LC3B (arrow) was detected using the purified polyclonal antibody.
anti-APG8b (MAP1LC3B) (Center), (Thr93), (Tyr99) antibody (2) APG8b (MAP1LC3B)-T93/Y99 (AP1802f) western blot analysis in mouse cerebellum tissue lysates (35 µg/lane).This demonstrates the MAP1LC3B antibody detected MAP1LC3B protein (arrow).
anti-APG8b (MAP1LC3B) (Center), (Thr93), (Tyr99) antibody (3) APG8b (MAP1LC3B)-T93/Y99 Antibody (Center) (ABIN388476) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining
Background publications Kabeya, Mizushima, Ueno et al.: "LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing." in: The EMBO journal, Vol. 19, Issue 21, pp. 5720-8, 2000 (PubMed).

Tanida, Tanida-Miyake, Komatsu et al.: "Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p." in: The Journal of biological chemistry, Vol. 277, Issue 16, pp. 13739-44, 2002 (PubMed).

Tanida, Tanida-Miyake, Nishitani et al.: "Murine Apg12p has a substrate preference for murine Apg7p over three Apg8p homologs." in: Biochemical and biophysical research communications, Vol. 292, Issue 1, pp. 256-62, 2002 (PubMed).

He, Dang, Dai et al.: "Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B." in: The Journal of biological chemistry, Vol. 278, Issue 31, pp. 29278-87, 2003 (PubMed).

Tanida, Ueno, Kominami: "Human light chain 3/MAP1LC3B is cleaved at its carboxyl-terminal Met121 to expose Gly120 for lipidation and targeting to autophagosomal membranes." in: The Journal of biological chemistry, Vol. 279, Issue 46, pp. 47704-10, 2004 (PubMed).

Stelzl, Worm, Lalowski et al.: "A human protein-protein interaction network: a resource for annotating the proteome...." in: Cell, Vol. 122, Issue 6, pp. 957-68, 2005 (PubMed).

Hosts (4)
Reactivities (4), (1)
Applications (3), (1), (1), (1)
Epitopes (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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