APG8b (MAP1LC3B) (Center, Thr93, Tyr99) antibody

Antigen: APG8b (MAP1LC3B)

Binding Site: Center, Thr93, Tyr99

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388476

Additional Information

Gene ID: 81631

UniProt: Q9GZQ8, NP_073729.1

Immunogen: This APG8b (MAP1LC3B)-T93/Y99 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 81-111 amino acids from the central region of human PMAT-Y198.

Isotype: Ig

Description: Other names: MAP1LC3B,MAP1ALC3

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This APG8b (MAP1LC3B)-T93/Y99 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 81-111 amino acids from the central region of human PMAT-Y198.

Molecular Weight: 14688 DA

Comments:

Background: The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure, Autophagy

Restrictions: For Research Use only

Images

Western blot analysis of APG8b (MAP1LC3B) Antibody (T93/Y99) (ABIN388476) in MCF-7 cell line lysates (35ug/lane). MAP1LC3B (arrow) was detected using the purified Pab.

APG8b (MAP1LC3B)-T93/Y99 (AP1802f) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the MAP1LC3B antibody detected MAP1LC3B protein (arrow).

APG8b (MAP1LC3B)-T93/Y99 Antibody (Center) (ABIN388476) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the APG8b (MAP1LC3B)-T93/Y99 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Publications

Product: Kabeya, Mizushima, Ueno et al.: "LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing." in: The EMBO journal, Vol. 19, Issue 21, pp. 5720-8, 2000 (PubMed).

Tanida, Tanida-Miyake, Komatsu et al.: "Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p." in: The Journal of biological chemistry, Vol. 277, Issue 16, pp. 13739-44, 2002 (PubMed).

Tanida, Tanida-Miyake, Nishitani et al.: "Murine Apg12p has a substrate preference for murine Apg7p over three Apg8p homologs." in: Biochemical and biophysical research communications, Vol. 292, Issue 1, pp. 256-62, 2002 (PubMed).

He, Dang, Dai et al.: "Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B." in: The Journal of biological chemistry, Vol. 278, Issue 31, pp. 29278-87, 2003 (PubMed).

Tanida, Ueno, Kominami: "Human light chain 3/MAP1LC3B is cleaved at its carboxyl-terminal Met121 to expose Gly120 for lipidation and targeting to autophagosomal membranes." in: The Journal of biological chemistry, Vol. 279, Issue 46, pp. 47704-10, 2004 (PubMed).

Stelzl, Worm, Lalowski et al.: "A human protein-protein interaction network: a resource for annotating the proteome." in: Cell, Vol. 122, Issue 6, pp. 957-68, 2005 (PubMed).