Cleaved LC3B antibody

Antigen: Cleaved LC3B

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388484

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Gene ID: 81631

Swiss-Prot: Q9GZQ8

Immunogen: This Cleaved LC3B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 96~125 amino acids from the C-terminal region of human cleaved-LC3 (APG8b).

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: MAP1LC3B, LC3B, MAP1A/1BLC3, MAP1LC3B

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide at the C-terminal cleavage site of human cleaved-LC3 (APG8b).

Molecular Weight: 14557 DA

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Immunofluorescence ~~ 1:10~50 Immunocytochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Cell Signaling, Cell Structure, Autophagy

Restrictions: For Research Use only

Images

Time course study of mouse leukaemic monocyte macrophage cells treated with U18666A, a drug that causes cholesterol and lipid storage in cells, thereby blocking fusion between late endosomes and lysosomes. Cleaved-LC3 (APG8b) antibody (ABIN388484) detected punctuate staining indicative of autophagic vacuole or phagosome structures. Data courtesy of Dr. Barry Boland, Department of Pharmacology, Oxford University.

SY5Y cells were pretreated with 5nM bafilomycin for 24hr and fixed in methanol (left panel) or 4% of paraformaldehyde (right panel). Treatment with ABIN388484 antibody at dilution 1:100. Data courtesy of Jianhui Zhu, MD, PhD & Charleen T. Chu, MD, PhD, University of Pittsburgh School of Medicine.

Publications

Publications: English, Chemali, Duron et al.: "Autophagy enhances the presentation of endogenous viral antigens on MHC class I molecules during HSV-1 infection." in: Nature immunology, Vol. 10, Issue 5, pp. 480-7, 2009 (PubMed).