ATG5 (C-term) antibody

Antigen: ATG5 (C-term)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388519

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Gene ID: 9474

Swiss-Prot: Q9H1Y0

Immunogen: This ATG5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 216~246 amino acids from the C-term of human APG5L.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: APG5, APG5-LIKE, APG5L, ASP, hAPG5, APG5 (autophagy 5, S. cerevisiae)-like, APG5 autophagy 5-like, ATG5 autophagy related 5-like, OTTHUMP00000017824, apoptosis specific protein, apoptosis-specific protein, autophagy protein 5

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide at the C-term of human APG5L.

Molecular Weight: 32447 DA

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Apoptosis/Necrosis, Cell Signaling, Cell Structure, Autophagy

Restrictions: For Research Use only

Images

Western blot analysis of APG5L Pab in Y79, mouse liver, and Hela tissue lysates(ABIN388519).

Cos7, HEK293, MEF, and Hela cells left to right, respectively. Data courtesy of Drs. Jiefei Geng and Dan Klionsky, University of Michigan.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.