ATG12 (N-term) antibody

Antigen: ATG12 (N-term)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388536

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Gene ID: 9140

Swiss-Prot: O94817

Immunogen: This ATG12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 44~73 amino acids from the N-term of human APG12L.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: APG12, APG12L, FBR93, HAPG12, APG12 autophagy 12-like, APG12-like, Apg12 (autophagy, yeast) homolog, autophagy-related protein 12

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide at the N-term of human APG12L.

Molecular Weight: 15113 DA

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Apoptosis/Necrosis, Cell Signaling, Cell Structure, Autophagy

Restrictions: For Research Use only

Images

Western blot analysis of anti-APG12L Pab (ABIN388536) in Hela cell lysate. APG12L (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with Autophagy APG12L antibody (N-term) (ABIN388536), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.