ATG16L antibody

Antigen: ATG16L

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388542

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Gene ID: 55054

Swiss-Prot: Q676U5

Immunogen: This ATG16L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 84~114 amino acids surrounding amino acid L92 of human APG16L.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: hCG_1817841, APG16L, ATG16L, FLJ00045, FLJ10035, FLJ10828, FLJ22677, IBD10, WDR30, APG16 autophagy 16-like, APG16-like 1, APG16L beta, ATG16 autophagy related 16-like protein 1, WD repeat domain 30, autophagy-related protein 16-1

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide surrounding amino acid L92 of human APG16L.

Molecular Weight: 68265 DA

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Cell Structure, Autophagy

Restrictions: For Research Use only

Images

Western blot of APG16L (L92) Pab (ABIN388542) in mouse brain tissue lysate.

Cos7, HEK293, MEF, and Hela cells, left to right, respectively. Data courtesy of Drs. Jiefei Geng and Dan Klionsky, University of Michigan.

Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with Autophagy APG16L antibody (L176), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.