UV Radiation Resistance Associated Gene (UVRAG) (C-Term) antibody

Antigen: UV Radiation Resistance Associated Gene (UVRAG)

Synonyms: p63, DHTX, Uvrag1, Uvragl, AI648770, BB124205, 9530039D02Rik, MGC56164, zgc:56164, UVRAG, uvrag, MGC81810, MGC131135

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388586

Additional Information

Alternative name: UVRAG

Gene ID: 7405

UniProt: Q9P2Y5

Immunogen: This UVRAG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 670~699 amino acids from the C-term of human UVRAG.

Isotype: Ig

Description: Other names: UV radiation resistance-associated gene protein, p63

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This UVRAG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 670~699 amino acids from the C-term of human UVRAG.

Molecular Weight: 78151 DA

Comments:

Background: UVRAG complements the ultraviolet sensitivity of xeroderma pigmentosum group C cells and encodes a protein with a C2 domain. The protein activates the Beclin1-PI(3)KC3 complex, promoting autophagy and suppressing the proliferation and tumorigenicity of human colon cancer cells. Chromosomal aberrations involving this gene are associated with left-right axis malformation and mutations in this gene have been associated with colon cancer.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunoprecipitation~~5ug/ml Immunohistochemistry~~1:50~100 immunofluorescence ~~ tested

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Cancer, Autophagy, Cell Structure

Restrictions: For Research Use only

Images

Immunoprecipitation and western blot analysis of anti-UVRAG Pab (ABIN388586) in 293T cells. UVRAG is immunoprecipitated (Lane 2) and detected in 293T cell transiently transfected with mouse UVRAG (Lane 1). Detection of endogenous UVRAG is shown in 293T cells (Lane 3) but is reduced by UVRAG siRNA transfection (Lane 4). Data courtesy of Dr. Hong-Gang Wang, Moffitt Cancer Center, Tampa, FL.

Immunofluorescence staining of Autophagy UVRAG antibody (ABIN388586) on Methanol-fixed HeLa cells. Data courtesy of Dr. Eeva-Liisa Eskelinen, University of Helsinki,Finland.

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with hUVRAG (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Perelman, Dafni, Naiman et al.: "Molecular cloning of a novel human gene encoding a 63-kDa protein and its sublocalization within the 11q13 locus." in: Genomics, Vol. 41, Issue 3, pp. 397-405, 1997 (PubMed).

Ionov, Nowak, Perucho et al.: "Manipulation of nonsense mediated decay identifies gene mutations in colon cancer Cells with microsatellite instability." in: Oncogene, Vol. 23, Issue 3, pp. 639-45, 2004 (PubMed).

Liang, Feng, Ku et al.: "Autophagic and tumour suppressor activity of a novel Beclin1-binding protein UVRAG." in: Nature cell biology, Vol. 8, Issue 7, pp. 688-99, 2006 (PubMed).