UV Radiation Resistance Associated Gene (UVRAG) (Leu133) antibody

Antigen: UV Radiation Resistance Associated Gene (UVRAG)

Synonyms: p63, DHTX, Uvrag1, Uvragl, AI648770, BB124205, 9530039D02Rik, MGC56164, zgc:56164, UVRAG, uvrag, MGC81810, MGC131135

Binding Site: Leu133

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388588

Additional Information

Alternative name: UVRAG

Gene ID: 7405

UniProt: Q9P2Y5

Immunogen: This UVRAG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 128~157 amino acids from the N-term (around L133) of human UVRAG.

Isotype: Ig

Description: Other names: UV radiation resistance-associated gene protein, p63

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This UVRAG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 128~157 amino acids from the N-term (around L133) of human UVRAG.

Molecular Weight: 78151 DA

Comments:

Background: UVRAG complements the ultraviolet sensitivity of xeroderma pigmentosum group C cells and encodes a protein with a C2 domain. The protein activates the Beclin1-PI(3)KC3 complex, promoting autophagy and suppressing the proliferation and tumorigenicity of human colon cancer cells. Chromosomal aberrations involving this gene are associated with left-right axis malformation and mutations in this gene have been associated with colon cancer.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunofluorescence~~ Tested

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Cancer, Autophagy, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-hUVRAG (Center) Pab (ABIN388588) in mouse liver tissue lysate. hUVRAG (Center)(arrow) was detected using the purified Pab.

Immunofluorescence staining of Autophagy UVRAG antibody (ABIN388588) on Methanol-fixed HeLa cells. Data courtesy of Dr. Eeva-Liisa Eskelinen, University of Helsinki,Finland.

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with hUVRAG (N-term L133), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Perelman, Dafni, Naiman et al.: "Molecular cloning of a novel human gene encoding a 63-kDa protein and its sublocalization within the 11q13 locus." in: Genomics, Vol. 41, Issue 3, pp. 397-405, 1997 (PubMed).

Ionov, Nowak, Perucho et al.: "Manipulation of nonsense mediated decay identifies gene mutations in colon cancer Cells with microsatellite instability." in: Oncogene, Vol. 23, Issue 3, pp. 639-45, 2004 (PubMed).

Liang, Feng, Ku et al.: "Autophagic and tumour suppressor activity of a novel Beclin1-binding protein UVRAG." in: Nature cell biology, Vol. 8, Issue 7, pp. 688-99, 2006 (PubMed).