Aconitase 2, Mitochondrial (ACO2) (Center) antibody

Antigen: Aconitase 2, Mitochondrial (ACO2)

Synonyms: ACONM, MGC20605, MGC33908, Aco3, Aco-2, D10Wsu183e, cb1017, wu:fa10e03, wu:fb69g04, wu:fc20c11, MGC84375, aconitase 2, F10M23.310, F10M23_310

Epitope: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388648

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: ACO2 (Center)

Gene ID: 50

Swiss-Prot: Q99798

Immunogen: This ACO2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 445~475 amino acids from the central region of human Aconitase.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: Aconitate hydratase, mitochondrial [Precursor], Citrate hydro-lyase, Aconitase, ACO2,

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the central region of human Aconitase.

Molecular Weight: 85425 DA

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-ACO2 Pab (ABIN388648) in mouse heart(left) and 293 (right) tissue lysates (35ug/lane). ACO2(arrow) was detected using the purified Pab (1:60 dilution).

Perfused isolated rat heart whole tissue lysate was lysed with either A) 50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.1% SDS, 0.5% Na-deoxycholate, 1 mM Na3VO4, 20 mM NaF, 1 mM PMSF, 5 v/v % protease inhibitor cocktail or B) T-PER Tissue Protein Extraction Reagent [# 785101. Pierce], containing 1mM Na3VO4, 20 mM NaF, 5 v/v % protease inhibitor cocktail (Sigma). PVDF membrane was incubated in primary Ab [rabbit polyclonal antibody against ACO2 (Center) (ABIN388648). Solution: 1:1000 diluted in 5% NFM TBS-T 0,05 for overnight (15 hrs) at 4 °. Data courtesy of Boglarka Laczy M.D., Division of Cardiovascular Disease, Dept. of Medicine, University of Alabama at Birmingham.

ACO2 (ABIN388648) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the Aconitase antibody detected the Aconitase protein (arrow).