ACO2 antibody (N-Term)

Catalog No. ABIN2786627

There are 2+ publications for this product available. The Rabbit Polyclonal anti-ACO2 antibody is suitable to detect ACO2 in samples from Human, Rat, Mouse, Dog, Cow, Saccharomyces cerevisiae, Guinea Pig, Horse and Rabbit. It has been validated for WB.

Quick Overview for ACO2 antibody (N-Term) (ABIN2786627)

Target: ACO2 (Aconitase 2, Mitochondrial (ACO2))

Reactivity: Human, Rat, Mouse, Dog, Cow, Saccharomyces cerevisiae, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACO2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ACO2 Antibody

Binding Specificity: N-Term

Purpose: ACO2 Antibody - N-terminal region

Sequence: LQFISSGLSK VAVPSTIHCD HLIEAQVGGE KDLRRAKDIN QEVYNFLATA

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 86%

Characteristics: This is a rabbit polyclonal antibody against ACO2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ACO2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP56301-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-ACO2 antibody (ABIN2786627)

Schauer, Freund, Prenni, Curthoys: "Proteomic profiling and pathway analysis of the response of rat renal proximal convoluted tubules to metabolic acidosis." in: American journal of physiology. Renal physiology, Vol. 305, Issue 5, pp. F628-40, (2013) (PubMed).

Freund, Prenni, Curthoys: "Response of the mitochondrial proteome of rat renal proximal convoluted tubules to chronic metabolic acidosis." in: American journal of physiology. Renal physiology, Vol. 304, Issue 2, pp. F145-55, (2013) (PubMed).

Target Details for ACO2

Target: ACO2 (Aconitase 2, Mitochondrial (ACO2))

Alternative Name: ACO2

Background:

Background Information: ACO2 belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification.The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: Aconitase 2, mitochondrial

Alternative Symbols: ICRD, OCA8, OPA9, ACONM, HEL-S-284

Protein Name: Aconitate hydratase, mitochondrial

Molecular Weight: 82kDa

Gene ID: 50

NCBI Accession: NP_001089

UniProt: Q99798