Bone gamma-Carboxyglutamate (Gla) Protein (BGLAP) (N-Term) antibody

Antigen: Bone gamma-Carboxyglutamate (Gla) Protein (BGLAP)

Synonyms: OC, BGP, PMF1, OG1, mOC-A, Bglap1, Bglap2, Bgp, Og2, mOC-B, Bgpr, Bgpra, BGLAP, bgp, LOC792433, ocn, LOC100286409

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388727

Additional Information

Alternative name: Osteocalcin

Gene ID: 632

UniProt: NP_954642

Immunogen: This Osteocalcin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9~39 amino acids from the N-terminal region of human osteocalcin.

Isotype: Ig

Description: Other names: Gamma-carboxyglutamic acid-containing protein, Bone Gla protein, BGP, BGLAP

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Osteocalcin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9~39 amino acids from the N-terminal region of human osteocalcin.

Molecular Weight: 10962 DA

Comments:

Background: Prior to the formation of calcified bone, noncollagenous proteins form in the extracellular bone matrix. Gamma-carboxyglutamic acid residues are formed by vitamin K, vitamin-D regulated calcium binding proteins containing residues of Gla. These residues are essential for the binding of calcium and constitue 1-2% of total bone protein. Osteocalcin itself binds strongly to apatite and calcium. Production of osteocalcin is expressed late in normal bone development and is characteristic of mature osteoblasts. Regular osteocalcin production has been shown to be linked to the p53 tumor suppressor gene. The p53 gene undergoes rearrangement in a high percentage of osteosarcomas, resulting in loss of its expression. The loss of p53 regulation inhibits further osteocalcin production. The absence of end-point differentiation in bone due to p53 rearrangements and lack of osteocalcin production may contribute to the maintenance of the tumorigenic phenotype in osteosarcomas.

Application Details

Application Notes: The suggested dilution will be: ELISA~~1:1,000 Western blot~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells, Cancer, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of Osteocalcin (arrow) using rabbit polyclonal Osteocalcin Antibody (N-term) (ABIN388727). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BGLAP gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

OSTC Antibody (C16) (ABIN388727) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the OSTC antibody detected the OSTC protein (arrow).

Publications

Product: Viereck, Siggelkow, Tauber et al.: "Differential regulation of Cbfa1/Runx2 and osteocalcin gene expression by vitamin-D3, dexamethasone, and local growth factors in primary human osteoblasts." in: Journal of cellular biochemistry, Vol. 86, Issue 2, pp. 348-56, 2002 (PubMed).

Willis, Loewy, Charlton-Kachigian et al.: "Regulation of osteocalcin gene expression by a novel Ku antigen transcription factor complex." in: The Journal of biological chemistry, Vol. 277, Issue 40, pp. 37280-91, 2002 (PubMed).