Thy-1 Cell Surface Antigen (THY1) (N-Term) antibody

Antigen: Thy-1 Cell Surface Antigen (THY1)

Synonyms: CD90, FLJ33325, CD7, MGC123140, wu:fb15c03, wu:fb18g05, zgc:123140, THY1, MGC128895, DKFZp459C1015, Thy-1

Epitope: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388803

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: THY1 (N-term)

Gene ID: 7070

Swiss-Prot: P04216

Immunogen: This THY1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human THY1.

Isotype: Ig

Description: Other names: Thy-1 membrane glycoprotein, Thy-1 antigen, CD90 antigen, CDw90

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human THY1.

Molecular Weight: 17935 DA

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Apoptosis/Necrosis, Stem Cells, Cell Structure, Neurology

Restrictions: For Research Use only

Images

The anti-THY1 N-term Pab (ABIN388803) is used in Western blot to detect THY1 in HL60 cell lysate.

Western blot analysis of anti-THY1 (N-term) Pab (ABIN388803) pre-incubated without(lane 1) and with(lane 2) blocking peptide (BP2050a) in T47D cell line lysate. THY1 (N-term)(arrow) was detected using the purified Pab (1:60 dilution).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.