SLUG (N-Term,Lys9) antibody

Antigen: SLUG

Binding Site: N-Term,Lys9

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388808

Additional Information

UniProt: O43623

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human SLUG.

Isotype: Ig

Description: Other names: Zinc finger protein SLUG, Neural crest transcription factor Slug, Snail homolog 2,SNAI2

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This SLUG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2~32 amino acids from the N-terminal region of human SLUG.

Comments:

Background: SLUG is a member of the Snail family of C2H2-type zinc finger transcription factors. This protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. Mutations in the gene encoding SLUG may be associated with sporadic cases of neural tube defects.

Application Details

Application Notes: The suggested dilution is: ELISA 1:1,000 Western blotting 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Research Area: Apoptosis/Necrosis, Stem Cells, Transcription Factors, Signaling

Restrictions: For Research Use only

Images

(LEFT)Western blot analysis of anti-SLUG Antibody (N-term K9) Pab (ABIN388808) in A2058 cell line lysates (35ug/lane). SLUG(arrow) was detected using the purified Pab. (RIGHT)Western blot analysis of SLUG Antibody (N-term K9) (ABIN388808) in HCSMC cell line lysates (35ug/lane).SLUG (arrow) was detected using the purified Pab.

Publications

Product: Hemavathy, Guru, Harris et al.: "Human Slug is a repressor that localizes to sites of active transcription." in: Molecular and cellular biology, Vol. 20, Issue 14, pp. 5087-95, 2000 (PubMed).

Hajra, Chen, Fearon: "The SLUG zinc-finger protein represses E-cadherin in breast cancer." in: Cancer research, Vol. 62, Issue 6, pp. 1613-8, 2002 (PubMed).

Sánchez-Martín, Rodríguez-García, Pérez-Losada et al.: "SLUG (SNAI2) deletions in patients with Waardenburg disease." in: Human molecular genetics, Vol. 11, Issue 25, pp. 3231-6, 2002 (PubMed).