SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) (N-Term) antibody

Antigen: SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1)

Synonyms: KIAA1625, mKIAA1625, 4930431E10Rik, SMURF1, Smurf1

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388828

Additional Information

Alternative name: SMURF1

Gene ID: 57154

UniProt: Q9HCE7

Immunogen: This SMURF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 74~104 amino acids from the N-terminal region of human SMURF1.

Isotype: Ig

Description: Other names: Smad ubiquitination regulatory factor 1, Ubiquitin--protein ligase SMURF1, Smad-specific E3 ubiquitin ligase 1, hSMURF1

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This SMURF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 74~104 amino acids from the N-terminal region of human SMURF1.

Molecular Weight: 86113 DA

Comments:

Background: Members of the transforming growth factor-beta (TGFB) family signal through type I and type II serine/threonine-kinase receptors, which in turn activate the SMAD signaling pathway. Bone morphogenetic protein (BMP) receptors target SMAD1, SMAD5, and SMAD8, whereas receptors for activin and TGFB (e.g., ACVR1 and TGFBR1, respectively) target SMAD2 and SMAD3. Phosphorylation of these receptor-regulated SMADs induces their association with the common-partner SMAD, SMAD4. Smurf1, a HECT domain E3 ubiquitin ligase, regulates cell polarity and protrusive activity and is required to maintain the transformed morphology and motility of a tumor cell. Atypical protein kinase C-zeta (PKC2), an effector of the Cdc42/Rac1-PAR6 polarity complex, recruits Smurf1 to cellular protrusions, where it controlled the local level of RhoA. Smurf1 thus links the polarity complex to degradation of RhoA in lamellipodia and filopodia to prevent RhoA signaling during dynamic membrane movements.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Cell Signaling, Protein Modifications, Cell Structure, Cancer, Cell Cycle

Restrictions: For Research Use only

Images

Western blot analysis of anti-SMURF1 Pab (ABIN388828) in mouse kidney tissue lysate (35ug/lane). SMURF1(arrow) was detected using the purified Pab.

C2C12 or 2T3 cells were treated with PBS or TNF (2.5-7.5 ng/ml) for 72 h. Smurf1 and actin protein levels were detected by Western blot (ABIN388828 Smurf1 N-term antibody).

Proteins were extracted from the metaphyseal region of 4-month-old TNF-over-expressing mice (TNF-Tg) and wild type (wt) littermates (left panel). PBS or TNF was injected (0.25 ug/injection, 3 times/day for 3 days) into the calvarial bones of wt mice. Proteins were extracted from the bones and analyzed by Western blotting for Smurf1 (ABIN388828 Smurf1 N-term antibody), Runx2 and alpha-actin protein levels (right panel).

Publications

Product: Zhu, Kavsak, Abdollah et al.: "A SMAD ubiquitin ligase targets the BMP pathway and affects embryonic pattern formation." in: Nature, Vol. 400, Issue 6745, pp. 687-93, 1999 (PubMed).

Ebisawa, Fukuchi, Murakami et al.: "Smurf1 interacts with transforming growth factor-beta type I receptor through Smad7 and induces receptor degradation." in: The Journal of biological chemistry, Vol. 276, Issue 16, pp. 12477-80, 2001 (PubMed).

Suzuki, Murakami, Fukuchi et al.: "Smurf1 regulates the inhibitory activity of Smad7 by targeting Smad7 to the plasma membrane." in: The Journal of biological chemistry, Vol. 277, Issue 42, pp. 39919-25, 2002 (PubMed).

Tajima, Goto, Yoshida et al.: "Chromosomal region maintenance 1 (CRM1)-dependent nuclear export of Smad ubiquitin regulatory factor 1 (Smurf1) is essential for negative regulation of transforming growth factor-beta signaling by Smad7." in: The Journal of biological chemistry, Vol. 278, Issue 12, pp. 10716-21, 2003 (PubMed).