SMURF1 antibody (C-Term)

Catalog No. ABIN388829

There is 1 publication for this product available. The Rabbit Polyclonal anti-SMURF1 antibody is suitable to detect SMURF1 in samples from Human. It has been validated for WB and IHC (p).

Quick Overview for SMURF1 antibody (C-Term) (ABIN388829)

Target: SMURF1 (SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMURF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB4103

Product Details anti-SMURF1 Antibody

Binding Specificity: AA 711-740, C-Term

Predicted Reactivity: M, X

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This SMURF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 711-740 amino acids from the C-terminal region of human SMURF1.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:500. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-SMURF1 antibody (ABIN388829)

Schwamborn, Khazaei, Püschel: "The interaction of mPar3 with the ubiquitin ligase Smurf2 is required for the establishment of neuronal polarity." in: The Journal of biological chemistry, Vol. 282, Issue 48, pp. 35259-68, (2007) (PubMed).

Target Details for SMURF1

Target: SMURF1 (SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1))

Alternative Name: SMURF1

Background: SMURF1 is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. This protein interacts with receptor-regulated SMADs specific for the BMP pathway, SMAD1 and SMAD5, in order to trigger their ubiquitination and degradation and thereby their inactivation.

Molecular Weight: 86114

Gene ID: 57154

NCBI Accession: NP_001186776, NP_065162, NP_851994

UniProt: Q9HCE7

Pathways: Regulation of Cell Size