Ubiquitin-Conjugating Enzyme E2S (UBE2S) (C-Term) antibody

Antigen: Ubiquitin-Conjugating Enzyme E2S (UBE2S)

Synonyms: EPF5, E2EPF, E2-EPF, AA409170, MGC101982, 0910001J09Rik, 6720465F12Rik, RGD1564746, UBE2S, ube2s, MGC114060

Epitope: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388858

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: E2EPF (C-term)

Gene ID: 27338

Swiss-Prot: Q16763

Immunogen: This E2EPF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 193~222 amino acids from the C-terminal region of human E2EPF.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: Ubiquitin-conjugating enzyme E2S, Ubiquitin-conjugating enzyme E2-24 kDa, Ubiquitin-protein ligase, Ubiquitin carrier protein, E2-EPF5

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human E2EPF.

Molecular Weight: 23845 DA

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Ubiquitin-related antibodies, Cell Signaling, Protein Modifications, Neurology, Cell Structure

Restrictions: For Research Use only

Images

The anti-E2EPF Pab (ABIN388858) is used in Western blot to detect E2EPF in Jurkat cell lysate.

Western blot analysis of E2EPF (arrow) using E2EPF Antibody (C-term) (ABIN388858). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the E2EPF gene (Lane 2) (Origene Technologies).

E2EPF Antibody (C-term) (ABIN388858)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of E2EPF Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.