Epidermal Growth Factor Receptor Pathway Substrate 15 (EPS15) (C-Term) antibody

Antigen: Epidermal Growth Factor Receptor Pathway Substrate 15 (EPS15)

Synonyms: anon-WO0118547.85, CG16932, DmelCG16932, EH1, eps-15, EPS-15, eps15, Eps15, i31, AF1P, AF-1P, MLLT5, 2410112D09Rik, RGD1307641, MGC151691

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388933

Additional Information

Alternative name: EPS15

Gene ID: 2060

UniProt: NP_001972

Immunogen: This EPS15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 848~877 amino acids from the C-terminal region of human EPS15.

Isotype: Ig

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This EPS15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 848~877 amino acids from the C-terminal region of human EPS15.

Molecular Weight: 98673 DA

Comments:

Background: EPS15 is involved in cell growth regulation, possibly via the regulation of mitogenic signals and control of cell proliferation. EPS15 also participates in the internalization of ligand-inducible receptors of the receptor tyrosine kinase (RTK) type, in particular EGFR. Potential interaction partners include AP2A2, STN2, EPN1, and CRK via its SH3-binding sites. EPS15 is ubiquitously expressed. Phosphorylation on Tyr-849 is involved in the internalization of EGFR. EPS15 is not required for membrane translocation after EGF treatment or for targeting to coated pits, but essential for a subsequent step in EGFR endocytosis. This protein is involved in a t(1,11)(p32,q23) chromosomal translocation in acute leukemias causing fusion to the trithorax (MLL or HRX) gene product which contains DNA-binding motifs resulting in a rogue activator protein. Structurally, EPS15 contains 2 EF-hand calcium-binding domains, 3 EH domains, and 2 ubiquitin-interacting motif (UIM) repeats.

Application Details

Application Notes: The suggested dilution will be: ELISA~~1:1,000 Western blotting~~1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Cell Structure, Protein Modifications, Cancer, Cell Cycle

Restrictions: For Research Use only

Images

Western blot analysis of anti-EPS15 Pab (ABIN388933) in HL60 cell line lysate (35ug/lane). EPS15 (arrow) was detected using the purified Pab.

Publications

Product: Matsuda, Ota, Tanimura et al.: "Interaction between the amino-terminal SH3 domain of CRK and its natural target proteins." in: The Journal of biological chemistry, Vol. 271, Issue 24, pp. 14468-72, 1996 (PubMed).

de Beer, Carter, Lobel-Rice et al.: "Structure and Asn-Pro-Phe binding pocket of the Eps15 homology domain." in: Science (New York, N.Y.), Vol. 281, Issue 5381, pp. 1357-60, 1998 (PubMed).

Chen, Fre, Slepnev et al.: "Epsin is an EH-domain-binding protein implicated in clathrin-mediated endocytosis." in: Nature, Vol. 394, Issue 6695, pp. 793-7, 1998 (PubMed).