Golgi-Associated, gamma Adaptin Ear Containing, ARF Binding Protein 1 (GGA1) (N-Term) antibody

Antigen: Golgi-Associated, gamma Adaptin Ear Containing, ARF Binding Protein 1 (GGA1)

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389018

Additional Information

Alternative name: GGA1

Gene ID: 26088

UniProt: Q9UJY5

Immunogen: This GGA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human GGA1.

Isotype: Ig

Description: Other names: ADP-ribosylation factor binding protein GGA1, Golgi-localized, gamma ear-containing, ARF-binding protein 1, Gamma-adaptin related protein 1

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This GGA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human GGA1.

Molecular Weight: 70384 DA

Comments:

Background: GGA1 is a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) protein family. Members of this family are ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins.

Application Details

Application Notes: The suggested dilution will be: ELISA~~1:1,000 Western blotting~~1:100~500 Immunofluorescence

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

The anti-GGA1 Pab (ABIN389018) is used in Western blot to detect GGA1 in mouse kidney tissue lysate (lane 1) and Jurkat cell lysate (lane 2).

A, N2a cells cotransfected with APP770 �V5 and GGA1 �myc or empty vector were immunostained for APP (Alexa488. green), GGA1 (Cy5. blue), and the Golgi marker GM130 (Cy3. red). B, The same transfection was stained for the endosomal marker EEA1 (Cy3). C, To assess cell-surface localization, APP695 �GFP and GGA1 �myc or empty vector were cotransfected and then immunostained with an Ab to the APP ectodomain on ice without permeabilization (Cy3). Cells were then fixed, permeabilized, and stained for GGA1 (Cy5). (J. Neurosci. 2006 Sep 27.26(39):9913-9922)

Publications

Product: Shiba, Takatsu, Nogi et al.: "Structural basis for recognition of acidic-cluster dileucine sequence by GGA1." in: Nature, Vol. 415, Issue 6874, pp. 937-41, 2002 (PubMed).

Zhu, Zhai, He et al.: "Crystal structure of the human GGA1 GAT domain." in: Biochemistry, Vol. 42, Issue 21, pp. 6392-9, 2003 (PubMed).

Zhai, He, Liu et al.: "The interaction of the human GGA1 GAT domain with rabaptin-5 is mediated by residues on its three-helix bundle." in: Biochemistry, Vol. 42, Issue 47, pp. 13901-8, 2003 (PubMed).