Heat Shock Factor Protein 1 (HSF1) (Sumoylation Site) antibody

Antigen: Heat Shock Factor Protein 1 (HSF1)

Synonyms: ARABIDOPSIS THALIANA CLASS A HEAT SHOCK FACTOR 1A, ARABIDOPSIS THALIANA HEAT SHOCK FACTOR 1, ATHSF1, ATHSFA1A, CLASS A HEAT SHOCK FACTOR 1A, DL4910C, FCAALL.107, HEAT SHOCK FACTOR, HEAT SHOCK FACTOR 1, HSF1, HSFA1A, hsf1, Hsf, xhsf1, HSTF1, AA960185

Binding Site: Sumoylation Site

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389069

Additional Information

Alternative name: HSF1

UniProt: Q00613

Immunogen: This antibody is generated from rabbits immunized with the KLH conjugated synthetic peptide PLVRVKEEPPSPPQSPR containing a predicted sumoylation site from the central region of human HSF1.

Isotype: Ig

Description: Other names: Heat shock factor protein 1, HSF 1, Heat shock transcription factor 1, HSTF 1, HSF1, HSTF1

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This HSF1 Sumoylation Site antibody is generated from rabbits immunized with the KLH conjugated synthetic peptide PLVRVKEEPPSPPQSPR containing a predicted sumoylation site from the central region of human HSF1.

Comments:

Background: Heat shock transcription factor 1 (HSF1) mediates the induction of heat shock protein gene expression in cells exposed to elevated temperature and other stress conditions. In response to stress, HSF1 acquires DNA-binding ability and localizes to nuclear stress granules. SUMO modification of HSF1 converts HSF1 to the DNA-binding form. HSF1 colocalizes with SUMO-1 in nuclear stress granules, which is prevented by mutation of the HSF1 lysine targeted for sumoylation.

Synonyms: ARABIDOPSIS THALIANA CLASS A HEAT SHOCK FACTOR 1A, ARABIDOPSIS THALIANA HEAT SHOCK FACTOR 1, ATHSF1, ATHSFA1A, CLASS A HEAT SHOCK FACTOR 1A, DL4910C, FCAALL.107, HEAT SHOCK FACTOR, HEAT SHOCK FACTOR 1, HSF1, HSFA1A, hsf1, Hsf, xhsf1, HSTF1, AA960185

Application Details

Application Notes: The suggested dilution will be: ELISA 1:1,000 Western Blot 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Research Area: Apoptosis/Necrosis, Cell Signaling, Protein Modifications, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of HSF1 polyclonal antibody (ABIN389069) in 293 cell line lysates. Non-sumoylated HSF1 (arrow) was detected using the purified Pab.

Western blot analysis of HSF1 (arrow) using rabbit polyclonal HSF1 Antibody (Sumo) (ABIN389069). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HSF1 gene.

Confocal immunofluorescent analysis of HSF1 Sumoylation Site Antibody (ABIN389069) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Publications

Product: Wang, Grammatikakis, Siganou et al.: "Regulation of molecular chaperone gene transcription involves the serine phosphorylation, 14-3-3 epsilon binding, and cytoplasmic sequestration of heat shock factor 1." in: Molecular and cellular biology, Vol. 23, Issue 17, pp. 6013-26, 2003 (PubMed).