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HSF1 antibody (AA 378-395) (Biotin)

HSF1 Reactivity: Mouse WB, ELISA, IHC, IP, IF, GS, ICC Host: Rat Monoclonal 10H8 Biotin
Catalog No. ABIN2484627
  • Target See all HSF1 Antibodies
    HSF1 (Heat Shock Factor Protein 1 (HSF1))
    Binding Specificity
    • 29
    • 15
    • 12
    • 12
    • 12
    • 11
    • 10
    • 9
    • 9
    • 9
    • 7
    • 7
    • 6
    • 6
    • 6
    • 6
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 378-395
    Reactivity
    • 193
    • 108
    • 57
    • 13
    • 11
    • 10
    • 7
    • 7
    • 6
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    Mouse
    Host
    • 179
    • 32
    • 7
    • 1
    Rat
    Clonality
    • 168
    • 50
    Monoclonal
    Conjugate
    • 118
    • 12
    • 11
    • 10
    • 8
    • 8
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This HSF1 antibody is conjugated to Biotin
    Application
    • 182
    • 110
    • 84
    • 65
    • 47
    • 43
    • 30
    • 28
    • 28
    • 27
    • 9
    • 6
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Gel Shift (GS), Immunocytochemistry (ICC)
    Specificity
    Detects ~85 kDa (unstressed cell lysates), and~95 kDa (heat shocked cell lysates).
    Cross-Reactivity
    Cow, Guinea Pig, Hamster, Human, Monkey, Mouse, Rabbit, Rat
    Purification
    Protein G Purified
    Immunogen
    Purified recombinant mouse HSF1 protein, with epitope mapping to amino acids 378-395
    Clone
    10H8
    Isotype
    IgG1
    Top Product
    Discover our top product HSF1 Primary Antibody
  • Application Notes
    • WB (1:1000)
    • IHC (1:1000)
    • ICC/IF (1:200)
    • optimal dilutions for assays should be determined by the user.
    Comment

    1 μg/ml of ABIN2484627 was sufficient for detection of HSF1 in 20 μg of heat shocked HeLa cell lysate by ECL immunoblot analysis using Goat anti-rat IgG: HRP as the secondary antibody

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Conjugated antibodies should be stored at 4°C
  • Target
    HSF1 (Heat Shock Factor Protein 1 (HSF1))
    Alternative Name
    HSF1 (HSF1 Products)
    Synonyms
    hsf1 antibody, Hsf antibody, xhsf1 antibody, AA960185 antibody, HSTF1 antibody, HSF1 antibody, HSF antibody, HSF1c antibody, hsf1a antibody, hsf1b antibody, zHSF1a antibody, zHSF1b antibody, hstf1 antibody, heat shock factor antibody, Heat Shock Factor antibody, heat shock factor 1 antibody, heat shock transcription factor 1 antibody, heat shock transcription factor 1 S homeolog antibody, hsf1 antibody, hsf-1 antibody, Hsf1 antibody, HSF1 antibody, hsf1.S antibody
    Background
    HSF1, or heat shock factor 1, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function (2). The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF) (3, 4, 5). Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the N-terminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization (6). There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transcriptionally active than the smaller beta form (7, 8). Various experiments have suggested that HFS2 may have roles in differentiation and development (9, 10, 11).
    Gene ID
    15499
    NCBI Accession
    NP_032322
    UniProt
    P38532
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