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Serine Palmitoyltransferase (SPTLC2) (C-Term) antibody
Western Blotting (WB), ELISA
|3 references available|
|Quantity||400 µL (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This SPTLC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from 532-562 human SPTLC2.|
|Description||Other names: Serine palmitoyltransferase 2, Long chain base biosynthesis protein 2, LCB 2, Serine-palmitoyl-CoA transferase 2, SPT 2, SPT2|
|Characteristics||Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This SPTLC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from 532-562 human SPTLC2.|
|Molecular Weight||62924 DA|
Background: Serine palmitoyltransferase (SPT) is the key enzyme in sphingolipid biosynthesis. It catalyzes the pyridoxal-5-prime-phosphate-dependent condensation of L-serine and palmitoyl-CoA to 3-oxosphinganine.
|Application Notes||The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Protein Modifications, Metabolism, Cell Structure|
|Restrictions||For Research Use only|
|The anti-SPTLC2 Pab (ABIN389107) is used in Western blot to detect SPTLC2 in mouse liver tissue lysate.|
Takeda, Yano, Eng et al.: "A molecular inventory of human pancreatic islets: sequence analysis of 1000 cDNA clones." in: Human molecular genetics, Vol. 2, Issue 11, pp. 1793-8, 1994 (PubMed).
Hillier, Lennon, Becker et al.: "Generation and analysis of 280,000 human expressed sequence tags." in: Genome research, Vol. 6, Issue 9, pp. 807-28, 1997 (PubMed).
Weiss, Stoffel: "Human and murine serine-palmitoyl-CoA transferase--cloning, expression and characterization of the key enzyme in sphingolipid synthesis." in: European journal of biochemistry / FEBS, Vol. 249, Issue 1, pp. 239-47, 1997 (PubMed).