Serine Palmitoyltransferase (SPTLC2) (C-Term) antibody

Antigen: Serine Palmitoyltransferase (SPTLC2)

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389107

Additional Information

Gene ID: 9517

UniProt: NP_004854

Immunogen: This SPTLC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from 532-562 human SPTLC2.

Isotype: Ig

Description: Other names: Serine palmitoyltransferase 2, Long chain base biosynthesis protein 2, LCB 2, Serine-palmitoyl-CoA transferase 2, SPT 2, SPT2

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This SPTLC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from 532-562 human SPTLC2.

Molecular Weight: 62924 DA

Comments:

Background: Serine palmitoyltransferase (SPT) is the key enzyme in sphingolipid biosynthesis. It catalyzes the pyridoxal-5-prime-phosphate-dependent condensation of L-serine and palmitoyl-CoA to 3-oxosphinganine.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Protein Modifications, Metabolism, Cell Structure

Restrictions: For Research Use only

Images

The anti-SPTLC2 Pab (ABIN389107) is used in Western blot to detect SPTLC2 in mouse liver tissue lysate.

Publications

Product: Takeda, Yano, Eng et al.: "A molecular inventory of human pancreatic islets: sequence analysis of 1000 cDNA clones." in: Human molecular genetics, Vol. 2, Issue 11, pp. 1793-8, 1994 (PubMed).

Hillier, Lennon, Becker et al.: "Generation and analysis of 280,000 human expressed sequence tags." in: Genome research, Vol. 6, Issue 9, pp. 807-28, 1997 (PubMed).

Weiss, Stoffel: "Human and murine serine-palmitoyl-CoA transferase--cloning, expression and characterization of the key enzyme in sphingolipid synthesis." in: European journal of biochemistry / FEBS, Vol. 249, Issue 1, pp. 239-47, 1997 (PubMed).