Acyl-CoA Synthetase Long-Chain Family Member 4 (ACSL4) (Center) antibody

Antigen: Acyl-CoA Synthetase Long-Chain Family Member 4 (ACSL4)

Synonyms: acsl4, MGC66186, zgc:66186, ACSL4

Epitope: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389113

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: ACSL4 (FACL4) (Center)

Gene ID: 2182

Swiss-Prot: ACSL4_HUMAN

Immunogen: This ACSL4 (FACL4) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 244~274 amino acids from the center region of human FACL4.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: Long-chain-fatty-acid--CoA ligase 4, Long-chain acyl-CoA synthetase 4, LACS4

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human FACL4.

Molecular Weight: 79188 DA

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Protein Modifications, Neurology, Cell Structure

Restrictions: For Research Use only

Images

The anti-FACL4 Pab (ABIN389113) is used in Western blot to detect FACL4 in RKO cell lysate (human colon cancer) treated with 10% FCS for 24 hrs. Data is kindly provided by Drs. M.G. Sciulli and P. Patrignani from the University of Chieti (Italy).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

FACL4-E251 (ABIN389113) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the FACL4 antibody detected the FACL4 protein (arrow).

Publications

Publications: Rodriguez, Bhat, Meloni et al.: "Intellectual disability, midface hypoplasia, facial hypotonia, and Alport syndrome are associated with a deletion in Xq22.3." in: American journal of medical genetics. Part A, Vol. 152A, Issue 3, pp. 713-7, 2010 (PubMed).