ACSL4 antibody (C-Term)

Catalog No. ABIN2854678

This Rabbit Polyclonal antibody specifically detects ACSL4 in WB, IF, ICC, IP and IHC (p). It exhibits reactivity toward Human. It has been mentioned in 3+ publications

Quick Overview for ACSL4 antibody (C-Term) (ABIN2854678)

Target: ACSL4 (Acyl-CoA Synthetase Long-Chain Family Member 4 (ACSL4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACSL4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ACSL4 Antibody

Binding Specificity: C-Term

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to FACL4 (acyl-CoA synthetase long-chain family member 4)
FACL4 antibody [C3], C-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FACL4. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:1000-1:5000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.44 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-ACSL4 antibody (ABIN2854678)

Ndiaye, Liu, Hall, Minogue, Morgan, Waugh: "Immunohistochemical staining reveals differential expression of ACSL3 and ACSL4 in hepatocellular carcinoma and hepatic gastrointestinal metastases." in: Bioscience reports, Vol. 40, Issue 4, (2020) (PubMed).

Garcia-Bermudez, Baudrier, Bayraktar, Shen, La, Guarecuco, Yucel, Fiore, Tavora, Freinkman, Chan, Lewis, Min, Inghirami, Sabatini, Birsoy: "Squalene accumulation in cholesterol auxotrophic lymphomas prevents oxidative cell death." in: Nature, Vol. 567, Issue 7746, pp. 118-122, (2019) (PubMed).

Radif, Ndiaye, Kalantzi, Jacobs, Hall, Minogue, Waugh: "The endogenous subcellular localisations of the long chain fatty acid-activating enzymes ACSL3 and ACSL4 in sarcoma and breast cancer cells." in: Molecular and cellular biochemistry, Vol. 448, Issue 1-2, pp. 275-286, (2018) (PubMed).

Target Details for ACSL4

Target: ACSL4 (Acyl-CoA Synthetase Long-Chain Family Member 4 (ACSL4))

Alternative Name: acyl-CoA synthetase long chain family member 4

Background: The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the mental retardation or Alport syndrome. Alternative splicing of this gene generates 2 transcript variants.

Cellular Localization: Mitochondrion outer membrane, Single-pass type III membrane protein , Peroxisome membrane, Single-pass type III membrane protein , Microsome membrane, Single-pass type III membrane protein , Endoplasmic reticulum membrane, Single-pass type III membrane pr

Molecular Weight: 79 kDa

Gene ID: 2182

UniProt: O60488