Olfactomedin 1 (OLFM1) (N-Term) antibody

Antigen: Olfactomedin 1 (OLFM1)

Synonyms: AMY, NOE1, OlfA, NOELIN1, Noe1, AW742568, Pancortin, Pancortin3, D2Sut1e, NOELIN-1, MGC132187, OLFM1, amy, noe1, olfa, noelin1, noelin-1

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389177

Additional Information

Alternative name: Olfm1

Gene ID: 10439

UniProt: Q99784

Immunogen: This Olfm1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 78~108 amino acids from the N-terminal region of human Olfm1.

Isotype: Ig

Description: Other names: Noelin, Neuronal olfactomedin-related ER localized protein, Olfactomedin-1, OLFM1, NOE1, NOEL1

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Olfm1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 78~108 amino acids from the N-terminal region of human Olfm1.

Molecular Weight: 55401 DA

Comments:

Background: Olfm1 shares extensive sequence similarity with the rat neuronal olfactomedin-related ER localized protein. While the exact function of this protein is not known, its abundant expression in brain suggests that it may have an essential role in nerve tissue.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells

Restrictions: For Research Use only

Images

Western blot analysis of Noelin-1(Olfm1) (arrow) using rabbit polyclonal Noelin-1(Olfm1) Antibody (N-term) (ABIN389177). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Noelin-1(Olfm1) gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with Olfm1 Antibody (N-term) (ABIN389177), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Yokoyama, Nishi, Yoshii et al.: "Identification and cloning of neuroblastoma-specific and nerve tissue-specific genes through compiled expression profiles." in: DNA research : an international journal for rapid publication of reports on genes and genomes, Vol. 3, Issue 5, pp. 311-20, 1997 (PubMed).

Yu, Andersson, Worley et al.: "Large-scale concatenation cDNA sequencing." in: Genome research, Vol. 7, Issue 4, pp. 353-8, 1997 (PubMed).

Liu, Qian, Gritsenko et al.: "Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry." in: Journal of proteome research, Vol. 4, Issue 6, pp. 2070-80, 2005 (PubMed).