Keratin 19 (KRT19) (C-Term) antibody

Antigen: Keratin 19 (KRT19)

Synonyms: K19, CK19, K1CS, MGC15366, EndoC, Krt1-19, AI663979, Krt-1.19, MGC25344, Ka19, MGC124534, MGC156553, k19, ck19, k1cs, krt9, krt15, MGC76282, KRT19, GK-19, MGC83069, LOC514812, MGC154386, DKFZp469N0613

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389327

Additional Information

Alternative name: KRT19

Gene ID: 3880

UniProt: P08727

Immunogen: This KRT19 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 343~373 amino acids surrounding S46 of human KRT19.

Isotype: Ig

Description: Other names: Keratin, type I cytoskeletal 19, Cytokeratin-19, CK-19, Keratin-19, K19, KRT19

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This KRT19 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 343~373 amino acids surrounding S46 of human KRT19.

Molecular Weight: 44092 DA

Comments:

Background: KRT19 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells, Cancer, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of KRT19 Antibody (C-term) (ABIN389327) in CEM cell line lysates (35ug/lane). KRT19 (arrow) was detected using the purified Pab.

KRT19 Antibody (C-term) (ABIN389327) IHC analysis in formalin fixed and paraffin embedded human Lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the KRT19 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Publications

Product: Yang, Xu, Shi et al.: "Cytokeratin 10 and cytokeratin 19: predictive markers for poor prognosis in hepatocellular carcinoma patients after curative resection." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 14, Issue 12, pp. 3850-9, 2008 (PubMed).

Maurer, Nelson, Ceceña et al.: "Contrasting expression of keratins in mouse and human embryonic stem cells." in: PLoS ONE, Vol. 3, Issue 10, pp. e3451, 2008 (PubMed).