Death-Domain Associated Protein (DAXX) (pSer213) antibody

Details for Product No. ABIN389550
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Antigen
Synonyms BING2, DAP6, EAP1, im:6905684, LOC100226911
Epitope
pSer213
(22), (17), (16), (16), (13), (13), (9), (5), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(176), (65), (52), (48), (37), (24), (5), (3)
Host
Rabbit
(149), (33)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(122), (70), (60), (35), (33), (31), (12), (7), (7), (5), (5), (1), (1), (1), (1)
Pubmed 5 references available
Catalog no. ABIN389550
Quantity 400 µL
Price
291.50 $   Plus shipping costs $45.00
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Immunogen This DAXX Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S213 of human DAXX.
Clone RB6929
Isotype Ig
Specificity This Phospho-DAXX-S213 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S213 of human DAXX.
Predicted Reactivity Mouse (Murine),Rat (Rattus)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name DAXX
Background DAXX is proposed to mediate activation of the JNK pathway and apoptosis via MAP3K5 in response to signaling from TNFRSF6 and TGFBR2. Interaction with HSPB1/HSP27 may prevent interaction with TNFRSF6 and MAP3K5 and block DAXX-mediated apoptosis. In contrast, in lymphoid cells JNC activation and TNFRSF6-mediated apoptosis may not involve DAXX. DAXX seems to regulate transcription in PML/POD/ND10 nuclear bodies together with PML and may influence TNFRSF6-dependent apoptosis thereby. This protein down-regulates basal and activated transcription, and it also seems to act as a transcriptional co-repressor and inhibits PAX3 and ETS1 through direct protein-protein interaction. DAXX modulates PAX5 activity. Its transcription repressor activity is modulated by recruiting it to subnuclear compartments like the nucleolus or PML/POD/ND10 nuclear bodies through interactions with MCSR1 and PML, respectively.
Synonyms: Death domain-associated protein 6, Daxx, hDaxx, Fas death domain-associated protein, ETS1 associated protein 1, EAP1, DAXX, BING2, DAP6,
Molecular Weight 81373 DA
Gene ID 1616
UniProt Q9UER7
Research Area Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Transcription Factors, Signaling
Application Notes WB = 1:1000, IHC = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 0.46 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Death-Domain Associated Protein (DAXX) (pSer213) antibody The anti-Phospho-DAXX-S213 Pab (ABIN389550) is used in Western blot to detect Phospho-DAXX-S213 in mouse thymus tissue lysate
anti-Death-Domain Associated Protein (DAXX) (pSer213) antibody (2) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
Background publications Beausoleil, Jedrychowski, Schwartz et al.: "Large-scale characterization of HeLa cell nuclear phosphoproteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 33, pp. 12130-5, 2004 (PubMed).

Gostissa, Morelli, Mantovani et al.: "The transcriptional repressor hDaxx potentiates p53-dependent apoptosis." in: The Journal of biological chemistry, Vol. 279, Issue 46, pp. 48013-23, 2004 (PubMed).

Chang, Lin, Fang et al.: "Daxx mediates the small ubiquitin-like modifier-dependent transcriptional repression of Smad4." in: The Journal of biological chemistry, Vol. 280, Issue 11, pp. 10164-73, 2005 (PubMed).

Greger, Katz, Ishov et al.: "The cellular protein daxx interacts with avian sarcoma virus integrase and viral DNA to repress viral transcription." in: Journal of virology, Vol. 79, Issue 8, pp. 4610-8, 2005 (PubMed).

Cantrell, Bresnahan: "Interaction between the human cytomegalovirus UL82 gene product (pp71) and hDaxx regulates immediate-early gene expression and viral replication." in: Journal of virology, Vol. 79, Issue 12, pp. 7792-802, 2005 (PubMed).

Hosts (149), (33)
Reactivities (176), (65), (52), (48), (37), (24), (5), (3)
Applications (122), (70), (60), (35), (33), (31), (12), (7), (7), (5), (5), (1), (1), (1), (1)
Conjugates (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6)
Epitopes (22), (17), (16), (16), (13), (13), (9), (5), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
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