NFkB antibody (pSer536)

Catalog No. ABIN389601

Cited in 2+ publications. The Rabbit Polyclonal anti-NFkB antibody (Clone RB5212) (ABIN389601) specifically detects NFkB in WB. The antibody is reactive with Human and Mouse samples.

Quick Overview for NFkB antibody (pSer536) (ABIN389601)

Target: NFkB (Nuclear Factor kappa B (NFkB))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFkB antibody is un-conjugated

Application: Western Blotting (WB)

Clone: RB5212

Product Details anti-NFkB Antibody

Binding Specificity: pSer536

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This NFKB Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S536 of human NFKB.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-NFkB antibody (ABIN389601)

Li, Lu, Yu, Han, Wang, Mao, Shen, Wang, Tang, Li, Song: "miR-221/222 promote cancer stem-like cell properties and tumor growth of breast cancer via targeting PTEN and sustained Akt/NF-κB/COX-2 activation." in: Chemico-biological interactions, Vol. 277, pp. 33-42, (2017) (PubMed).

Wang, Cai, Shao, Siddiqui, Palanisamy, Li, Ren, Ayala, Ittmann: "Activation of NF-{kappa}B by TMPRSS2/ERG Fusion Isoforms through Toll-Like Receptor-4." in: Cancer research, Vol. 71, Issue 4, pp. 1325-33, (2011) (PubMed).

Target Details for NFkB

Target: NFkB (Nuclear Factor kappa B (NFkB))

Alternative Name: NFKB

Background: NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T, R is an A or G purine, and Y is a C or T pyrimidine).

Molecular Weight: 60219

Gene ID: 5970

NCBI Accession: NP_001138610, NP_001230913, NP_068810

UniProt: Q04206

Pathways: Ubiquitin Proteasome Pathway, S100 Proteins