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Antibodies
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anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (S213) antibody
SMAD, Mothers Against DPP Homolog 3 (SMAD3) (S213) antibody
| Antigen |
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| Synonyms |
MADH3, JV15-2, HSPC193, HsT17436, MGC60396, DKFZp586N0721, DKFZp686J10186 |
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Epitope
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pSer425 (6), Center D201 (1), Center N206 (1), S204 (1), S208 (1), pS423 pS425 (1), pSer204 (1), pSer208 (1), pSer213 (1), pSer423, pSer425 (1)
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| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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 Alternatives Western Blotting (WB), Immunohistochemistry (IHC), ELISA
Western Blotting (WB) (55), Immunofluorescence (IF) (36), Immunohistochemistry (IHC) (30), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (29), Enzyme Immunoassay (EIA) (27), ELISA (19), Flow Cytometry (FACS) (18), Immunoprecipitation (IP) (10), Dot Blot (Dot) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN389657 |
| Quantity |
0.1 mg (0.25 mg/ml) |
| Price |
383.50 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
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Alternative name
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Phospho-SMAD3-S213
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Gene ID
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4088
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Swiss-Prot
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P84022
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Immunogen
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This Phospho-SMAD3-S213 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S213 of human SMAD3.
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Isotype
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Ig (Matching secondary antibodies)
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Description
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Other names: Mothers against decapentaplegic homolog 3, SMAD 3, Mothers against DPP homolog 3, Mad3, hMAD-3, JV15-2, hSMAD3, SMAD3, MADH3,
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Specificity
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S213 of human SMAD3.
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Molecular Weight
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48081 DA
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Application Notes
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The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100
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Concentration
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0.25 mg/ml
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Purification
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Purified
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Buffer
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylate
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Storage
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Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles
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Research Area
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Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cancer, Cell Cycle, Transcription Factors, Signaling, Cell Structure
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Restrictions
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For Research Use only
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Alternatives for antigen "SMAD, Mothers Against DPP Homolog 3 (SMAD3)", type "Antibodies"
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Hosts
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Rabbit (84), Mouse (20)
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Reactivities
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Human (96), Rat (Rattus) (57), Mouse (Murine) (52), Cow (Bovine) (19), Chicken (15)
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Applications
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Western Blotting (WB) (55), Immunofluorescence (IF) (36), Immunohistochemistry (IHC) (30), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (29), Enzyme Immunoassay (EIA) (27), ELISA (19), Flow Cytometry (FACS) (18), Immunoprecipitation (IP) (10), Dot Blot (Dot) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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pSer425 (6), Center D201 (1), Center N206 (1), S204 (1), S208 (1), pS423 pS425 (1), pSer204 (1), pSer208 (1), pSer213 (1), pSer423, pSer425 (1)
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