Microtubule-Associated Protein 1 Light Chain 3 gamma (MAP1LC3C) (pSer12) antibody

Details for Product No. ABIN389699
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Antigen
Synonyms ATG8J, LC3C, MAP1LC3C, MGC154878
Epitope
pSer12
(3), (2), (2), (1), (1), (1)
Reactivity
Human
(26), (13), (12), (12), (12), (1), (1)
Host
Rabbit
(21), (3), (1), (1)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Dot Blot (DB)
(12), (10), (8), (6), (4), (2), (1), (1)
Pubmed 5 references available
Catalog no. ABIN389699
Quantity 400 µL
Price
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Immunogen This LC3C Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S12 of human LC3C.
Clone RB10838-RB16769
Isotype Ig
Specificity This Phospho-LC3C-S12 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 1~30 amino acids surrounding S12 of human LC3 (APG8a). Patent pending.
Predicted Reactivity Cow (Bovine),Mouse (Murine),Rat (Rattus)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name LC3C
Background MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3a is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole).
Synonyms: MAP1LC3C, microtubule-associated protein 1 light chain 3 gamma, LC3C, MAP1LC3C
Molecular Weight 14141 DA
Gene ID 84557
UniProt Q5JWU0
Research Area Cell Signaling, Cell Structure, Autophagy, Phospho-specific antibodies
Application Notes WB = 1:1000, DB = 1:500
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Microtubule-Associated Protein 1 Light Chain 3 gamma (MAP1LC3C) (pSer12) antibody Immunoblots of phosphorylated LC3 (phospho-LC3) in CHO cell culture. LC3 and LC3 S12A mutant vectors were transfected into CHO cells. The cell lysates were separated with SDS-PAGE and blotted with anti-phospho-LC3 S12 antibody. LC3 = microtubule-associated protein light chain-3. S12A = replacement of the amino acid position 12 serine of LC3 with alanine. WT = wildtype LC3-transfected cell lysates. S12A = LC3 S12A mutant-transfected cell lysates. Empty vector = vector with no LC3 gene. Molecular size: LC3-I = 16kDa, and LC3-II = 14 kDa
anti-Microtubule-Associated Protein 1 Light Chain 3 gamma (MAP1LC3C) (pSer12) antibody (2) Dot blot analysis of Phospho-LC3 (APG8a) - S12 Antibody (ABIN389699) and Nonphospho-LC3 (APG8a) Antibody on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 µg/mL.
anti-Microtubule-Associated Protein 1 Light Chain 3 gamma (MAP1LC3C) (pSer12) antibody (3) Immunoblots of SH-SY5Y cells treated with rapamycin for 1 h was probed with ABIN389699. The data shows that treatment with rapamycin showed no significant change in level of LC3.
Background publications He, Dang, Dai et al.: "Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B." in: The Journal of biological chemistry, Vol. 278, Issue 31, pp. 29278-87, 2003 (PubMed).

Tanida, Ueno, Kominami: "LC3 conjugation system in mammalian autophagy." in: The international journal of biochemistry & cell biology, Vol. 36, Issue 12, pp. 2503-18, 2004 (PubMed).

Shintani, Klionsky: "Autophagy in health and disease: a double-edged sword." in: Science (New York, N.Y.), Vol. 306, Issue 5698, pp. 990-5, 2004 (PubMed).

Lum, DeBerardinis, Thompson: "Autophagy in metazoans: cell survival in the land of plenty." in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 439-48, 2005 (PubMed).

Baehrecke: "Autophagy: dual roles in life and death?" in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 505-10, 2005 (PubMed).

Hosts (21), (3), (1), (1)
Reactivities (26), (13), (12), (12), (12), (1), (1)
Applications (12), (10), (8), (6), (4), (2), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (3), (2), (2), (1), (1), (1)
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