Microtubule-Associated Protein 1 Light Chain 3 gamma (MAP1LC3C) (pSer12) antibody

Antigen: Microtubule-Associated Protein 1 Light Chain 3 gamma (MAP1LC3C)

Synonyms: LC3C, MAP1LC3C, MGC154878

Binding Site: pSer12

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Dot Blot (Dot), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389699

Additional Information

Alternative name: LC3C

Gene ID: 84557

UniProt: Q5JWU0

Immunogen: This Phospho-LC3C-S12 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 1~30 amino acids surrounding S12 of human LC3 (APG8a). Patent pending.

Isotype: Ig

Description: Other names: MAP1LC3C, microtubule-associated protein 1 light chain 3 gamma, LC3C, MAP1LC3C

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Phospho-LC3C-S12 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 1~30 amino acids surrounding S12 of human LC3 (APG8a). Patent pending.

Molecular Weight: 14141 DA

Comments:

Background: MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3a is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole).

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Dot Blot ~~ 1:100~500 Western Blot ~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein A affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Cell Structure, Autophagy, Phospho-specific antibodies

Restrictions: For Research Use only

Images

Immunoblots of phosphorylated LC3 (phospho-LC3) in CHO cell culture. LC3 and LC3 S12A mutant vectors were transfected into CHO cells. The cell lysates were separated with SDS-PAGE and blotted with anti-phospho-LC3 S12 antibody. LC3 = microtubule-associated protein light chain-3. S12A = replacement of the amino acid position 12 serine of LC3 with alanine. WT = wildtype LC3-transfected cell lysates. S12A = LC3 S12A mutant-transfected cell lysates. Empty vector = vector with no LC3 gene. Molecular size: LC3-I = 16kDa, and LC3-II = 14 kDa

Dot blot analysis of Phospho-LC3 (APG8a) - S12 Antibody (ABIN389699) and Nonphospho-LC3 (APG8a) Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Immunoblots of SH-SY5Y cells treated with rapamycin for 1 h was probed with ABIN389699. The data shows that treatment with rapamycin showed no significant change in level of LC3.

Publications

Product: He, Dang, Dai et al.: "Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B." in: The Journal of biological chemistry, Vol. 278, Issue 31, pp. 29278-87, 2003 (PubMed).

Tanida, Ueno, Kominami: "LC3 conjugation system in mammalian autophagy." in: The international journal of biochemistry & cell biology, Vol. 36, Issue 12, pp. 2503-18, 2004 (PubMed).

Shintani, Klionsky: "Autophagy in health and disease: a double-edged sword." in: Science (New York, N.Y.), Vol. 306, Issue 5698, pp. 990-5, 2004 (PubMed).

Lum, DeBerardinis, Thompson: "Autophagy in metazoans: cell survival in the land of plenty." in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 439-48, 2005 (PubMed).

Baehrecke: "Autophagy: dual roles in life and death?" in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 505-10, 2005 (PubMed).