Phospho-LC3C-S12 antibody

Antigen: Phospho-LC3C-S12

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Dot Blot (Dot), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389699

Additional Information

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Gene ID: 84557

Swiss-Prot: Q5JWU0

Immunogen: This Phospho-LC3C-S12 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 1~30 amino acids surrounding S12 of human LC3 (APG8a). Patent pending.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: MAP1LC3C, microtubule-associated protein 1 light chain 3 gamma, LC3C, MAP1LC3C

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S12 of human LC3 (APG8a). Patent pending.

Molecular Weight: 14141 DA

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Dot Blot ~~ 1:100~500 Western Blot ~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein A affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Cell Structure, Autophagy, Phospho-specific antibodies

Restrictions: For Research Use only

Images

Immunoblots of phosphorylated LC3 (phospho-LC3) in CHO cell culture. LC3 and LC3 S12A mutant vectors were transfected into CHO cells. The cell lysates were separated with SDS-PAGE and blotted with anti-phospho-LC3 S12 antibody. LC3 = microtubule-associated protein light chain-3. S12A = replacement of the amino acid position 12 serine of LC3 with alanine. WT = wildtype LC3-transfected cell lysates. S12A = LC3 S12A mutant-transfected cell lysates. Empty vector = vector with no LC3 gene. Molecular size: LC3-I = 16kDa, and LC3-II = 14 kDa

Dot blot analysis of Phospho-LC3 (APG8a) - S12 Antibody (ABIN389699) and Nonphospho-LC3 (APG8a) Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Immunoblots of SH-SY5Y cells treated with rapamycin for 1 h was probed with ABIN389699. The data shows that treatment with rapamycin showed no significant change in level of LC3.