X-Linked Inhibitor of Apoptosis (XIAP) (N-Term) antibody

Antigen: X-Linked Inhibitor of Apoptosis (XIAP)

Synonyms: API3, ILP1, MIHA, XLP2, BIRC4, XIAP, birc4, xiap, MGC107868, xxiap

Epitope: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390070

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: XIAP (BIRC4) (N-term)

Gene ID: 331

Swiss-Prot: P98170

Immunogen: This XIAP (BIRC4) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 5~34 amino acids from the N-terminal region of human BIRC4.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: Baculoviral IAP repeat-containing protein 4, Inhibitor of apoptosis protein 3, X-linked inhibitor of apoptosis protein, X-linked IAP, IAP-like protein, HILP, API3, IAP3, XIAP

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human BIRC4.

Molecular Weight: 56684 DA

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Apoptosis/Necrosis, Cancer, Cell Structure

Restrictions: For Research Use only

Images

The anti-BIRC4 N-term Pab (ABIN390070) is used in Western blot to detect BIRC4 in mouse liver tissue lysate.

Western blot analysis of anti-BIRC4 Antibody (N-term) (ABIN390070) in Ramos cell line lysates (35ug/lane). BIRC4(arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with BIRC4 antibody (N-term) (ABIN390070), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.