CRY2 antibody (C-Term)

Catalog No. ABIN390080

The Rabbit Polyclonal anti-CRY2 antibody has been validated for WB and IHC (p). It is suitable to detect CRY2 in samples from Human and Mouse. There is 1 publication available.

Quick Overview for CRY2 antibody (C-Term) (ABIN390080)

Target: CRY2 (Cryptochrome 2 (Photolyase-Like) (CRY2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CRY2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB1846

Product Details anti-CRY2 Antibody

Binding Specificity: AA 564-593, C-Term

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This Cry2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 564-593 amino acids from the C-terminal region of human Cry2.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Expiry Date: 6 months

References for anti-CRY2 antibody (ABIN390080)

Wang, Wang, Liu, Liu, Tay, Walsh, Yang, Wu: "CRISPR/Cas9 mediated genome editing of Helicoverpa armigera with mutations of an ABC transporter gene HaABCA2 confers resistance to Bacillus thuringiensis Cry2A toxins." in: Insect biochemistry and molecular biology, Vol. 87, pp. 147-153, (2017) (PubMed).

Target Details for CRY2

Target: CRY2 (Cryptochrome 2 (Photolyase-Like) (CRY2))

Alternative Name: Cry2

Background: Various biochemical, physiological and behavioural processes display circadian rhythms controlled by an internal biological clock. The central ?gears?driving this clock appear to be composed of an autoregulatory transcription/posttranslation-based feedback loop. Cryptochrome 1 (CRY1) and 2 (CRY2) are DNA-binding flavoproteins that bear some homology to blue-light receptors and photolyases. In Drosophila, CRY is a photoreceptor for the circadian clock where it binds to the clock component TIM in a light-dependent fashion and blocks its function. Mammalian CRY1 and CRY2 function via light-independent interactions with circadian genes CLOCK and BMAL1, as well as with PER1, PER2, and TIM. They seem to act as light-independent components of the circadian clock and likely regulate Per1 transcriptional cycling via interactions with both the activator and its feedback inhibitors. Mutant mice not expressing the Cry1 or Cry2 protein display accelerated and delayed periodicity of locomotor activity, respectively. It appears that the combination of both proteins working together is essential to synchronize the organism to circadian phases. A critical balance between Cry1 and Cry2 is required for proper clock function, in complete darkness, double-mutant mice present with instantaneous arrhythmicity, indicating the absence of an internal circadian clock.

Molecular Weight: 66947

Gene ID: 1408

NCBI Accession: NP_001120929, NP_066940

UniProt: Q49AN0

Pathways: Response to Water Deprivation, Protein targeting to Nucleus