Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14) (N-Term) antibody

Antigen: Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14)

Synonyms: MMP14, MMP-14, mmp-x1, mtmmp1, mt1-mmp, MGC69200

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390138

Additional Information

Alternative name: MMP14

Gene ID: 4323

UniProt: NP_004986

Immunogen: This MMP14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human MMP14.

Isotype: Ig

Description: Other names: Matrix metalloproteinase-14 [Precursor], MMP-14, EC 3.4.24.80, Membrane-type matrix metalloproteinase 1, MT-MMP 1, MTMMP1, Membrane-type-1 matrix metalloproteinase, MT1-MMP, MT1MMP, MMP-X1, MMP14

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This MMP14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human MMP14.

Molecular Weight: 65883 DA

Comments:

Background: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP14 seems to specifically activate progelatinase A, and may thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. Expression is significant in stromal cells of colon, breast, and head and neck.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells, Protein Modifications, Cell Structure, Cancer

Restrictions: For Research Use only

Images

The anti-MMP14 N-term Antibody (ABIN390138) is used in Western blot to detect MMP14 in A375 lysate.

Western blot analysis of MMP14 (arrow) using rabbit polyclonal MMP14 Antibody (N-term) (ABIN390138). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MMP14 gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Will, Hinzmann: "cDNA sequence and mRNA tissue distribution of a novel human matrix metalloproteinase with a potential transmembrane segment." in: European journal of biochemistry / FEBS, Vol. 231, Issue 3, pp. 602-8, 1995 (PubMed).

Okada, Bellocq, Rouyer et al.: "Membrane-type matrix metalloproteinase (MT-MMP) gene is expressed in stromal cells of human colon, breast, and head and neck carcinomas." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 7, pp. 2730-4, 1995 (PubMed).

Sato, Takino, Okada et al.: "A matrix metalloproteinase expressed on the surface of invasive tumour cells." in: Nature, Vol. 370, Issue 6484, pp. 61-5, 1994 (PubMed).