Matrix Metallopeptidase 24 (Membrane-inserted) (MMP24) (Center) antibody

Antigen: Matrix Metallopeptidase 24 (Membrane-inserted) (MMP24)

Synonyms: MMP25, MT-MMP5, MT5-MMP, MT5MMP, MTMMP5, AU040325, Mt5-mmp

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390145

Additional Information

Alternative name: MMP24

Gene ID: 10893

UniProt: NP_006681

Immunogen: This MMP24 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human MMP24.

Isotype: Ig

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This MMP24 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human MMP24.

Molecular Weight: 73231 DA

Comments:

Background: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, MMP24 is a member of the membrane-type MMP (MT-MMP) subfamily, each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 by cleavage. The gene has previously been referred to as MMP25 but has been renamed matrix metalloproteinase 24 (MMP24).

Application Details

Application Notes: The suggested dilution will be: ELISA~~1:1,000 Western Blot~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells, Protein Modifications, Cell Structure, Cancer

Restrictions: For Research Use only

Images

Western blot analysis of MMP24 Antibody (Center) (ABIN390145) in HL60 cell line lysates (35ug/lane). MMP24 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Llano, Pendás, Freije et al.: "Identification and characterization of human MT5-MMP, a new membrane-bound activator of progelatinase a overexpressed in brain tumors." in: Cancer research, Vol. 59, Issue 11, pp. 2570-6, 1999 (PubMed).

Nagase, Woessner: "Matrix metalloproteinases." in: The Journal of biological chemistry, Vol. 274, Issue 31, pp. 21491-4, 1999 (PubMed).