Matrix Metallopeptidase 25 (MMP25) (C-Term) antibody

Antigen: Matrix Metallopeptidase 25 (MMP25)

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390146

Additional Information

Alternative name: MMP25

Gene ID: 64386

UniProt: NP_071913

Immunogen: This MMP25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human MMP25.

Isotype: Ig

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This MMP25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human MMP25.

Molecular Weight: 62554 DA

Comments:

Background: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, MMP25 is a member of the membrane-type MMP (MT-MMP) subfamily, each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. In addition, it is thought that this protein is activated intracellularly by furin-like enzymes. This protein activates MMP2 by cleavage. The gene has previously been referred to as MMP20 but has been renamed matrix metalloproteinase 25 (MMP25).

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells, Protein Modifications, Cell Structure, Cancer

Restrictions: For Research Use only

Images

The anti-MMP25 C-term Antibody (ABIN390146) is used in Western blot to detect MMP25 in mouse liver tissue lysate (lane 1) and HL60 cell lysate (lane 2) lysate.

Publications

Product: Nagase, Woessner: "Matrix metalloproteinases." in: The Journal of biological chemistry, Vol. 274, Issue 31, pp. 21491-4, 1999 (PubMed).

Matsuda, Itoh, Koshikawa et al.: "Clusterin, an abundant serum factor, is a possible negative regulator of MT6-MMP/MMP-25 produced by neutrophils." in: The Journal of biological chemistry, Vol. 278, Issue 38, pp. 36350-7, 2003 (PubMed).