Sirtuin 3 (SIRT3) (C-Term) antibody

Antigen: Sirtuin 3 (SIRT3)

Synonyms: SIR2L3, MGC158774, zgc:158774, SIRT3

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390178

Additional Information

Alternative name: SIRT3

Gene ID: 23410

UniProt: Q9NTG7

Immunogen: This SIRT3 antibody was raised against a peptide selected from the C-terminal region of human Sirt3.

Isotype: Ig

Description: Other names: SIR2-like protein 3, mSIR2L3, NAD-dependent deacetylase sirtuin-3,Sir2l3, Sirt3

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This polyclonal antibody was raised against a peptide selected from the C-terminal region of human Sirt3.

Molecular Weight: 43573 DA

Application Details

Application Notes: ELISA~~1:1,000 Western blotting~~1:50~100 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Cell Structure

Restrictions: For Research Use only

Images

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Western blot analysis of SIRT3 antibody (ABIN390178) in Y79 cell line lysates (35ug/lane). SIRT3 (arrow) was detected using the purified Pab.

Sirt3 Antibody (G265) (ABIN390178) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the Sirt3 antibody detected the Sirt3 protein (arrow).

Publications

Pillai, Sundaresan, Kim et al.: "Exogenous NAD blocks cardiac hypertrophic response via activation of the SIRT3-LKB1-AMP-activated kinase pathway." in: The Journal of biological chemistry, Vol. 285, Issue 5, pp. 3133-44, 2010 (PubMed).

Kawamura, Uchijima, Horike et al.: "Sirt3 protects in vitro-fertilized mouse preimplantation embryos against oxidative stress-induced p53-mediated developmental arrest." in: The Journal of clinical investigation, 2010 (PubMed).