WNT1 Inducible Signaling Pathway Protein 1 (WISP1) (Center) antibody

Antigen: WNT1 Inducible Signaling Pathway Protein 1 (WISP1)

Synonyms: CCN4, WISP1c, WISP1i, WISP1tc, WISP-1, WISP1, si:ch211-38f10.2

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390187

Additional Information

Alternative name: WISP1

Gene ID: 8840

UniProt: WISP1_HUMAN

Immunogen: This WISP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human WISP1.

Isotype: Ig

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This WISP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human WISP1.

Molecular Weight: 40331 DA

Comments:

Background: Wisp1 is a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. Wisp1 may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. It is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. The encoded protein binds to decorin and biglycan, two members of a family of small leucine-rich proteoglycans present in the extracellular matrix of connective tissue, and possibly prevents the inhibitory activity of decorin and biglycan in tumor cell proliferation. It also attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase. It is 83% identical to the mouse protein at the amino acid level.

Application Details

Application Notes: The suggested dilution will be: ELISA~~1:1,000 Western Blot~~ 1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Cancer, Cell Cycle, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of WISP1 Antibody (Center) (ABIN390187) in HL60 cell line lysates (35ug/lane). WISP1 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Xie, Nakachi, Wang et al.: "Elevated levels of connective tissue growth factor, WISP-1, and CYR61 in primary breast cancers associated with more advanced features." in: Cancer research, Vol. 61, Issue 24, pp. 8917-23, 2001 (PubMed).

Su, Overholtzer, Besser et al.: "WISP-1 attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase." in: Genes & development, Vol. 16, Issue 1, pp. 46-57, 2002 (PubMed).

Hocevar, Mou, Rennolds et al.: "Regulation of the Wnt signaling pathway by disabled-2 (Dab2)." in: The EMBO journal, Vol. 22, Issue 12, pp. 3084-94, 2003 (PubMed).