|+1 404 474 4654|
|+1 888 205 9894 (TF)|
WNT1 Inducible Signaling Pathway Protein 1 (WISP1) (Center) antibody
|Synonyms||CCN4, WISP1c, WISP1i, WISP1tc, WISP-1, WISP1, si:ch211-38f10.2|
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), ELISA
|3 references available|
|Quantity||0.1 mg (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This WISP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human WISP1.|
|Characteristics||Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This WISP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human WISP1.|
|Molecular Weight||40331 DA|
Background: Wisp1 is a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. Wisp1 may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. It is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. The encoded protein binds to decorin and biglycan, two members of a family of small leucine-rich proteoglycans present in the extracellular matrix of connective tissue, and possibly prevents the inhibitory activity of decorin and biglycan in tumor cell proliferation. It also attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase. It is 83% identical to the mouse protein at the amino acid level.
|Application Notes||The suggested dilution will be: ELISA~~1:1,000 Western Blot~~ 1:100~500 Immunohistochemistry~~1:50~100|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Cell Signaling, Cancer, Cell Cycle, Cell Structure|
|Restrictions||For Research Use only|
Xie, Nakachi, Wang et al.: "Elevated levels of connective tissue growth factor, WISP-1, and CYR61 in primary breast cancers associated with more advanced features." in: Cancer research, Vol. 61, Issue 24, pp. 8917-23, 2001 (PubMed).
Su, Overholtzer, Besser et al.: "WISP-1 attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase." in: Genes & development, Vol. 16, Issue 1, pp. 46-57, 2002 (PubMed).
Hocevar, Mou, Rennolds et al.: "Regulation of the Wnt signaling pathway by disabled-2 (Dab2)." in: The EMBO journal, Vol. 22, Issue 12, pp. 3084-94, 2003 (PubMed).
|Hosts||Rabbit (30), Goat (4), Mouse (1)|
|Reactivities||Human (31), Mouse (Murine) (3)|
|Applications||ELISA (16), Western Blotting (WB) (16), Immunofluorescence (IF) (14), Immunohistochemistry (IHC) (6), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1)|
|Conjugates||Biotin (3), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||C-Term (2), Internal Region (2), AA 185-200 (1), Center (1), Middle Region (1)|