DNA-Damage-Inducible Transcript 4 (DDIT4) (N-Term) antibody

Antigen: DNA-Damage-Inducible Transcript 4 (DDIT4)

Synonyms: CHOP, CEBPZ, CHOP10, GADD153, MGC4154, Dig2, REDD1, REDD-1, RTP801, FLJ20500, RP11-442H21.1, dig2, Rtp801, AA415483, 5830413E08Rik, redd1, MGC64453, MGC76013, MGC139459

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390207

Additional Information

Alternative name: DDIT4

Gene ID: 54541

UniProt: Q9NX09

Immunogen: This DDIT4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27~57 amino acids from the C-terminal region of human DDIT4.

Isotype: Ig

Description: Other names: Protein regulated in development and DNA damage response 1, REDD-1, HIF-1 responsive protein RTP801

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This DDIT4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27~57 amino acids from the C-terminal region of human DDIT4.

Molecular Weight: 25240 DA

Comments:

Background: REDD1 is a novel transcriptional target of p53 induced following DNA damage. During embryogenesis, REDD1 expression mirrors the tissue-specific pattern of the p53 family member p63, and TP63 null embryos show virtually no expression of REDD1, which is restored in mouse embryo fibroblasts following p63 expression. In differentiating primary keratinocytes, TP63 and REDD1 expression are coordinately downregulated, and ectopic expression of either gene inhibits in vitro differentiation. REDD1 appears to function in the regulation of reactive oxygen species (ROS), TP63 null fibroblasts have decreased ROS levels and reduced sensitivity to oxidative stress, which are both increased following ectopic expression of either TP63 or REDD1. Thus, REDD1 encodes a shared transcriptional target that implicates ROS in the p53-dependent DNA damage response and in p63-mediated regulation of epithelial differentiation.

Application Details

Application Notes: The suggested dilution is: ELISA 1:1,000 Western blotting 1:50~100 Immunohistochemistry ~~ 1:50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Transcription Factors, Signaling

Restrictions: For Research Use only

Images

Western blot analysis of DDIT4 Antibody (N-term) (ABIN390207) in Jurkat cell line lysates (35ug/lane). DDIT4 (arrow) was detected using the purified Pab (1:60 dilution).

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with DDIT4 antibody (N-term)(ABIN390207), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Ellisen, Ramsayer, Johannessen et al.: "REDD1, a developmentally regulated transcriptional target of p63 and p53, links p63 to regulation of reactive oxygen species." in: Molecular cell, Vol. 10, Issue 5, pp. 995-1005, 2002 (PubMed).