HIR Histone Cell Cycle Regulation Defective Homolog A (S. Cerevisiae) (HIRA) (Center) antibody

Antigen: HIR Histone Cell Cycle Regulation Defective Homolog A (S. Cerevisiae) (HIRA)

Synonyms: TUP1, DGCR1, TUPLE1, HIRA, wu:fb97b07, wu:fi43b08, si:ch211-188g24.3

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390223

Additional Information

Alternative name: HIRA

Gene ID: 7290

UniProt: P54198

Immunogen: This HIRA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 439~468 amino acids from the Center region of human HIRA.

Isotype: Ig

Description: Other names: TUP1-like enhancer of split protein 1, DGCR1, HIR, TUPLE1

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This HIRA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 439~468 amino acids from the Center region of human HIRA.

Molecular Weight: 111835 DA

Comments:

Background: HIRA is a histone chaperone that preferentially places the variant histone H3.3 in nucleosomes. Orthologs of this protein in yeast, flies, and plants are necessary for the formation of transcriptionally silent heterochomatin. It plays an important role in the formation of the senescence-associated heterochromatin foci. These foci likely mediate the irreversible cell cycle changes that occur in senescent cells. It is considered the primary candidate protein in some haploinsufficiency syndromes such as DiGeorge syndrome, and insufficient production of the gene may disrupt normal embryonic development.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: ion of Alternative Lengthening of Telomeres-Associated PML Bodies Requires HIRA but Not ASF1a. PLoS ONE 6(2): e17036. doi:10.1371/journal.pone.0017036. HIRA Antibody (Center) -

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Cycle, Signaling, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-HIRA Antibody (Center) (ABIN390223) in HL60 cell line lysates (35ug/lane). HIRA (arrow) was detected using the purified Pab (1:60 dilution).

Publications

Product: Zhang, Chen, Adams: "Molecular dissection of formation of senescence-associated heterochromatin foci." in: Molecular and cellular biology, Vol. 27, Issue 6, pp. 2343-58, 2007 (PubMed).