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HIRA antibody (AA 421-729)

HIRA Reactivity: Human, Mouse ICC, IF, WB Host: Mouse Monoclonal WC119-2H11 unconjugated
Catalog No. ABIN6971732
$605.00
Plus shipping costs $45.00
100 μg
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  • Target See all HIRA Antibodies
    HIRA (HIR Histone Cell Cycle Regulation Defective Homolog A (HIRA))
    Binding Specificity
    • 15
    • 9
    • 9
    • 6
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 421-729
    Reactivity
    • 47
    • 32
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human, Mouse
    Host
    • 54
    • 11
    Mouse
    Clonality
    • 54
    • 11
    Monoclonal
    Conjugate
    • 32
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This HIRA antibody is un-conjugated
    Application
    • 37
    • 24
    • 13
    • 13
    • 6
    • 5
    • 5
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
    Characteristics
    HIRA (DGCR1, TUP1) is a histone chaperone that preferentially places the variant Histone H3.3 in nucleosomes, replacing canonical Histone H3 during the cell cycle. HIRA is required for the periodic repression of histone gene transcription during the cell cycle and when complexed with ASF1a is involved in the formation of the senescence-associated heterochromatin foci. HIRA antibody (mAb) (Clone WC119.2H11) was raised in a Mouse host. It has been validated for use in Immunocytochemistry, Immunofluorescence and Western blot, it has been shown to react with Human and Mouse samples.
    Purification
    Protein G Chromatography
    Immunogen
    This HIRA antibody was raised against a GST-fusion protein containing residues 421-729 of human HIRA.
    Clone
    WC119-2H11
    Isotype
    IgG
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Buffer
    Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
  • Target
    HIRA (HIR Histone Cell Cycle Regulation Defective Homolog A (HIRA))
    Alternative Name
    HIRA (HIRA Products)
    Synonyms
    HIRA, wu:fb97b07, wu:fi43b08, si:ch211-188g24.3, AA138857, D16Ertd95e, N28177, Tuple1, DGCR1, TUP1, TUPLE1, dgcr1, hira-A, tup1, tuple1, homolog of histone chaperone HIRA, histone cell cycle regulator, histone cell cycle regulator a, protein HIRA, HIR histone cell cycle regulation defective homolog A (S. cerevisiae), HIRA, histone cell cycle regulator S homeolog, histone chaperone HIRA-like protein, Tuple1/HirA, HIRA, hira, LOC100052845, LOC100352472, LOC100407742, LOC100450459, LOC100467668, LOC100581135, LOC486424, Hira, LOC732736, hira.S
    Background
    HIRA (DGCR1, TUP1) is a histone chaperone that preferentially places the variant Histone H3.3 in nucleosomes, replacing canonical Histone H3 during the cell cycle. HIRA is required for the periodic repression of histone gene transcription during the cell cycle and when complexed with ASF1a is involved in the formation of the senescence-associated heterochromatin foci.
    Molecular Weight
    120 kDa
    NCBI Accession
    NP_003316
    Pathways
    Chromatin Binding
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