Poly (ADP-Ribose) Polymerase Family, Member 6 (PARP6) (C-Term, Tyr503) antibody

Antigen: Poly (ADP-Ribose) Polymerase Family, Member 6 (PARP6)

Synonyms: MGC131971, 1700119G14Rik, 2310028P13Rik, 3110038K10Rik, C030013N01Rik, RGD1310937, PARP6, MGC166370, DKFZp459B2362

Binding Site: C-Term, Tyr503

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390248

Additional Information

Alternative name: Parp6

Gene ID: 56965

UniProt: Q2NL67

Immunogen: This Parp6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 496~525 amino acids from the C-terminal region of human PARP6.

Isotype: Ig

Description: Other names: Poly [ADP-ribose] polymerase 6, PARP-6, PARP6

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Parp6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 496~525 amino acids from the C-terminal region of human PARP6.

Molecular Weight: 71115 DA

Comments:

Background: Poly(ADP-ribosyl)ation is an immediate DNA-damage-dependent post-translational modification of histones and other nuclear proteins that contributes to the survival of injured proliferating cells. Poly(ADP-ribose) polymerases (PARPs) now constitute a large family of 18 proteins, encoded by different genes and displaying a conserved catalytic domain in which PARP-1 (113 kDa), the founding member, and PARP-2 (62 kDa) are so far the sole enzymes whose catalytic activity has been shown to be immediately stimulated by DNA strand breaks. A large repertoire of sequences encoding novel PARPs now extends considerably the field of poly(ADP-ribosyl)ation reactions to various aspects of the cell biology including cell proliferation and cell death. Some of these new members interact with each other, share common partners and common subcellular localizations suggesting possible fine tuning in the regulation of this post-translational modification of proteins.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Translation Factors, Cell Cycle, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of Parp6 (arrow) using rabbit polyclonal Parp6 Antibody (C-term) (ABIN390248). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Parp6 gene.

Parp6 Antibody (C-term) (ABIN390248) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the Parp6 antibody detected the Parp6 protein (arrow).

Publications

Product: Amé, Spenlehauer, de Murcia: "The PARP superfamily." in: BioEssays : news and reviews in molecular, cellular and developmental biology, Vol. 26, Issue 8, pp. 882-93, 2004 (PubMed).