Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1) (N-Term) antibody

Antigen: Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1)

Synonyms: Q99, AGO1, EIF2C, GERP95, DKFZp686M13167, Ago, Ago-1, Ago1, Dm Ago1, ago1-1, anon-WO0257455.29, dAGO1, l(2)04845, l(2)4845, l(2)k00208, l(2)k08121, DmelCG6671, CG6671, GB12654, ARGONAUTE 1, T1N15.2, T1N15_2, dsim_GLEANR_9718, DsimGD25729, GD25729, EIF2C1

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390473

Additional Information

Alternative name: AGO1

Gene ID: 26523

UniProt: Q9UL18

Immunogen: This AGO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 34~64 amino acids from the N-terminal region of human AGO1.

Isotype: Ig

Description: Other names: Protein argonaute-1, Argonaute1, hAgo1, Eukaryotic translation initiation factor 2C 1, eIF-2C 1, eIF2C 1, Putative RNA-binding protein Q99, AGO1, EIF2C1

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This AGO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 34~64 amino acids from the N-terminal region of human AGO1.

Molecular Weight: 97214 DA

Comments:

Background: AGO1 is a member of the Argonaute family of proteins which play a role in RNA interference. The protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing.

Synonyms: Q99, AGO1, EIF2C, GERP95, DKFZp686M13167, Ago, Ago-1, Ago1, Dm Ago1, ago1-1, anon-WO0257455.29, dAGO1, l(2)04845, l(2)4845, l(2)k00208, l(2)k08121, DmelCG6671, CG6671, GB12654, ARGONAUTE 1, T1N15.2, T1N15_2, dsim_GLEANR_9718, DsimGD25729, GD25729, EIF2C1

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Transcription Factors, Signaling

Restrictions: For Research Use only

Images

Western blot analysis of AGO1 Antibody (N-term) (ABIN390473) in K562 cell line lysates (35ug/lane). AGO1 (arrow) was detected using the purified Pab.

AGO1 Antibody (N-term) (ABIN390473) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the AGO1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Publications

Product: Horikawa, Wood, Yang et al.: "Single nucleotide polymorphisms of microRNA machinery genes modify the risk of renal cell carcinoma." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 14, Issue 23, pp. 7956-62, 2008 (PubMed).

Weinmann, Höck, Ivacevic et al.: "Importin 8 is a gene silencing factor that targets argonaute proteins to distinct mRNAs." in: Cell, Vol. 136, Issue 3, pp. 496-507, 2009 (PubMed).