Exportin 1, CRM1 Homolog (Yeast) (XPO1) (C-Term) antibody

Antigen: Exportin 1, CRM1 Homolog (Yeast) (XPO1)

Synonyms: Xpo1, 18.m06600, XPO1, LOC100220104

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390636

Additional Information

Alternative name: XPO1

Gene ID: 7514

UniProt: O14980

Immunogen: This XPO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 824~854 amino acids from the C-terminal region of human XPO1.

Isotype: Ig

Description: Other names: Exportin-1, Exp1, Chromosome region maintenance 1 protein homolog, CRM1, XPO1

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This XPO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 824~854 amino acids from the C-terminal region of human XPO1.

Molecular Weight: 123386 DA

Comments:

Background: XPO1 mediates leucine-rich nuclear export signal (NES)-dependent protein transport. Exportin 1 specifically inhibits the nuclear export of Rev and U snRNAs. It is involved in the control of several cellular processes by controlling the localization of cyclin B, MPAK, and MAPKAP kinase 2. This protein also regulates NFAT and AP-1.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cancer, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of XPO1 antibody (C-term) (ABIN390636) in mouse bladder tissue lysates (35ug/lane). XPO1 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human brain tissue reacted with XPO1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Flow cytometric analysis of widr cells using XPO1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Dong, Biswas, Chook: "Structural basis for assembly and disassembly of the CRM1 nuclear export complex." in: Nature structural & molecular biology, Vol. 16, Issue 5, pp. 558-60, 2009 (PubMed).