Mastermind-Like 3 (Drosophila) (MAML3) (C-Term) antibody

Antigen: Mastermind-Like 3 (Drosophila) (MAML3)

Synonyms: GDN, MAM2, CAGH3, ERDA3, MAM-2, TNRC3

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390676

Additional Information

Alternative name: MAML3

Gene ID: 55534

UniProt: Q96JK9

Immunogen: This MAML3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 645~675 amino acids from the C-terminal region of human MAML3.

Isotype: Ig

Description: Other names: Mastermind-like protein 3, Mam-3, KIAA1816, MAML3

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This MAML3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 645~675 amino acids from the C-terminal region of human MAML3.

Molecular Weight: 121780 DA

Comments:

Background: MAML3 acts as a transcriptional coactivator for NOTCH proteins. The protein has been shown to amplify NOTCH-induced transcription of HES1.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cancer, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of MAML3 antibody (C-term) (ABIN390676) in Hela cell line lysates (35ug/lane). MAML3 (arrow) was detected using the purified Pab.

MAML3 Antibody (C-term) (ABIN390676) immunohistochemistry analysis in formalin fixed and paraffin embedded human lymphnode followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MAML3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

MAML3 Antibody (C-term) (ABIN390676) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Lin, Oyama, Nagase et al.: "Identification of new human mastermind proteins defines a family that consists of positive regulators for notch signaling." in: The Journal of biological chemistry, Vol. 277, Issue 52, pp. 50612-20, 2002 (PubMed).

Tonon, Modi, Wu et al.: "t(11;19)(q21;p13) translocation in mucoepidermoid carcinoma creates a novel fusion product that disrupts a Notch signaling pathway." in: Nature genetics, Vol. 33, Issue 2, pp. 208-13, 2003 (PubMed).